Glucose Dehydrogenase from E. coli, Recombinant

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Cat No.

NATE-1902

Description

In enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction:beta-D-glucose + NAD (P)+↔ D-glucono-1,5-lactone + NAD (P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

Abbr

GDH, Recombinant (E. coli)

Alias

GDH

Source

E. coli

Applications

This enzyme is useful for determination of glucose.

Appearance

Lyophilized

Enzyme Commission Number

EC 1.1.1.47

CAS_No

9028-53-9

Contaminants

as GlcDH2 activity = 100 %)
NADH oxidase: <0.01 %

Molecular Weight

ca. 126,000; Subunit molecular weight : ca. 31,500.

pH Stability

5.0 - 10.0 (with 3M NaCl)

Michaelis Constant

D-Glucose: 3.7 mM;
NAD+: 0.06 mM;
NADP+: 0.02 mM.

Specificity

D-Glucose: 100 %;
D-Maltose: 1.1 %;
D-Galactose: 0.1 %;
D-Xylose: 3.0 %;
D-Fructose: 0.3 %;
D-Mannose: 4.8 %;
D-Arabinose: 0 %;
Trehalose: 0 %;
D-Lactose: 1.3 %;
D-Sucrose: 0 %;
2-Deoxy-D-Glucose: 100 %;
D-Glucose-1-Phosphate: 0 %;
D-Glucose-6-Phosphate: 0 %;
D-Sorbitol: 0 %;

Unit Definition

One unit of activity is defined as the amount of GlcDH2 that forms 1 μmol of NADH per minute at 37 °C.

Optimum pH

8.5

Thermal stability

No significant decrease in activity up to 70 °C. (with 3M NaCl and 0.1% BSA).

Storage

Stable at -20 °C for at least one year.

Synonyms

EC 1.1.1.47; D-glucose dehydrogenase (NAD (P)+); hexose phosphate dehydrogenase; β-D-glucose:NAD (P)+ 1-oxidoreductase; glucose 1-dehydrogenase; Glucose dehydrogenase; 9028-53-9

Specific Activity

more than 900 U/mg protein

Reaction

D-Glucose + NAD(P)+ ←→ D-Glucono-δ-lactone + NAD(P)H + H+

"Glucose Dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1794	Glucose Dehydrogenase (Crude Enzyme)	EC 1.1.1.47	9028-53-9	E. coli	Inquiry
DIA-191	Native Microorganism Glucose Dehydrogenase (NAD(P)-dependent)	EC 1.1.1.47	9028-53-9	Microorganism	Inquiry
DIA-192	Native Microorganism Glucose Dehydrogenase (PQQ-dependent)	EC 1.1.5.2	81669-60-5	Microorganism	Inquiry
NATE-0305	Native Pseudomonas sp. Glucose dehydrogenase	EC 1.1.1.47	9028-53-9	Pseudomonas sp.	Inquiry

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