HIV-2 Protease, Recombinant

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Cat No.

NATE-1661

Description

HIV-2 Protease, an aspartyl protease (retropepsin), is essential for the life-cycle of HIV-2 sub-type virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytically released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients, the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells. HIV-2 has been found to be less pathogenic than HIV-1. The mechanism of HIV-2 is not clearly defined, nor the difference from HIV-1, however the transmission rate is much lower in HIV-2 than HIV-1.

Abbr

HIV-2 Protease, Recombinant

Source

E. coli

Form

Liquid

Enzyme Commission Number

EC 3.4.23.-

Molecular Weight

38.3 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31.5 kDa during SEC and SDS-PAGE analyses.

Purity

> 85%

Stability

Stable for at least 6 months as supplied. It can be further diluted to 0.5-1 mg/ml with 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol, and stored at –80°C in aliquots. Do not keep the enzyme at 4°C or -20°C for extended time. Avoid repeated freezing and thawing cycles.

Storage

Store at -80°C

Synonyms

HIV-2 retropepsin; HIV-2 Protease (PR2); cd05482

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1662	HIV-1 Protease, Recombinant	EC 3.4.23.-		E. coli	Inquiry
NATE-0344	HIV Protease Mutant, Recombinant	EC 3.4.23.16	144114-21-6	E. coli	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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