Keratan sulfate Endo-N-acetylglucosaminidase 111A from Bacillus circulans

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Cat No.

NATE-1940

Description

BcKsh111A (GH111), E.C. number 3.2.1.-, is an endo-β -N-acetylglucosaminidase from Bacillus circulans. Recombinant BcKsh111A (GH111), purified from Escherichia coli, is a single domain family 111 Glycoside Hydrolase (GH111).

Source

E. coli

Species

Bacillus circulans

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

EC 3.2.1.-

Molecular Weight

69,07 kDa

Purity

>90% as judged by SDS-PAGE

Concentration

0.5 mg/mL

Specificity

BcKsh111A (GH111) hydrolyses cartilage keratan sulfate and cornea keratan sulfate.

Optimum temperature

55 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

endo-β -N-acetylglucosaminidase; single domain family 111 Glycoside Hydrolase; Keratan sulfate Endo-N-acetylglucosaminidase 111A; Keratan sulfate Endo-N-acetylglucosaminidase; GH111

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