Native Bacillus stearothermophilus Phosphofructokinase

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Cat No.

NATE-0551

Description

Phosphofructokinase is a kinase enzyme that phosphorylates fructose 6-phosphate in glycolysis. The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes. One enzyme that utilizes this reaction is phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, a key regulatory step in the glycolytic pathway. PFK exists as a homotetramer in bacteria and mammals (where each monomer possesses 2 similar domains) and as an octomer in yeast (where there are 4 alpha-(PFK1) and 4 beta-chains (PFK2), the latter, like the mammalian monomers, possessing 2 similar domains[3]). This protein may use the morpheein model of allosteric regulation.

Abbr

PFK, Native (Bacillus stearothermophilus)

Alias

PFKWII; PFK

Source

Bacillus stearothermophilus

Applications

Useful for enzymatic determiantion of fructose-6-phosphate

Appearance

White to pale yellow powder

Product Overview

Is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.

Form

Freeze dried powder

Enzyme Commission Number

EC 2.7.1.11

Activity

> 250 U/mg

CAS_No

9001-80-3

Contaminants

NADPH oxidase < 0.01%; ATPase < 0.005%

Molecular Weight

72 kDa (gel filtration); 35 kDa (SDS‒PAGE)

Isoelectric point

pH 5.9

pH Stability

6.0-10.0 (37°C, 60 mins)

Michaelis Constant

D‒Fructose‒6‒phosphate (D‒F‒6‒P) 5.8 mM (at 37°C); ATP 0.07 mM (at 37°C)

Unit Definition

One unit is defined as the amount of enzyme which converts 1 μmole of fructose‒6‒phosphate to Fructose‒1,6 ‒bisphosphate per minute at 37°C under the conditions specified in the assay procedure.

Optimum pH

Activators

Mg2+

Thermal stability

Stable at 55°C and below (pH 8.5, 30 mins)

Storage

Storage at-20°C in the presence of a desiccant is recommended.

Synonyms

PFKWII; EC 2.7.1.11; PFK; phosphofructokinase; 6-phosphofructokinase; Phosphofructokinase I; Phosphohexokinase

"PFK" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-403	Native Phosphofructokinase from Thermophillic bacteria	EC 2.7.1.11	9001-80-3	Thermophillic bacteria	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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