Native Bovine Phosphodiesterase 3',5'-Cyclic Nucleotide Activator-deficient

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Cat No.

NATE-0514

Description

Hydrolyzes the 3',5'-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5'-monophosphate.

Abbr

PDE, Native (Bovine)

Alias

PDE

Source

Bovine heart

Species

Bovine

Applications

Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold. For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.

Package

Package size based on activated units

Form

lyophilized powder (contains imidazole buffer salts and magnesium sulfate)

Enzyme Commission Number

EC 3.1.4.17

Activity

>0.1 units/mg protein (with added activator)

CAS_No

9040-59-9

Molecular Weight

mol wt ~60 kDa

Unit Definition

One unit will hydrolyze 1.0 μmole of 3':5',-cyclic-AMP to 5'-AMP per min at pH 7.5 at 30°C.

Storage

−20°C

Buffer

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0°C. However, the activator deficient activity may increase up to 200%. If stored at 4°C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.

Warnings

Crude preparation which has been stripped of the protein activator, calmodulin. Protein determined by Warburg-Christian unless specified otherwise.

Function

calcium-and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; protein binding

Synonyms

cyclic 3',5'-mononucleotide phosphodiesterase; PDE; cyclic 3',5'-nucleotide phosphodiesterase; cyclic 3',5'-phosphodiesterase; 3',5'-nucleotide phosphodiesterase; 3':5'-cyclic nucleotide 5'-nucleotidohydrolase; 3',5'-cyclonucleotide phosphodiesterase; cyclic nucleotide phosphodiesterase; 3', 5'-cyclic nucleoside monophosphate phosphodiesterase; 3':5'-monophosphate phosphodiesterase (cyclic CMP); cytidine 3':5'-monophosphate phosphodiesterase (cyclic CMP); cyclic 3',5-nucleotide monophosphate phosphodiesterase; nucleoside 3',5'-cyclic phosphate diesterase; nucleoside-3',5-monophosphate phosphodiesterase; EC 3.1.4.17

"PDE" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0511	Native Bothrops atrox Phosphodiesterase I	EC 3.1.4.1	9025-82-5	Bothrops atrox	Inquiry
NATE-0512	Native Crotalus adamanteus venom Phosphodiesterase I	EC 3.1.4.1	9025-82-5	Crotalus adamanteus venom	Inquiry
NATE-0513	Native Crotalus atrox (Western Diamondback Rattlesnake) Phosphodiesterase I	EC 3.1.4.1	9025-82-5	Crotalus atrox (Western Diamondback Rattlesnake)	Inquiry
NATE-0515	Native Bovine Phosphodiesterase, 3',5'-cyclic-nucleotide-specific	EC 3.1.4.17	9040-59-9	Bovine brain	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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