Native Elizabethkingia meningoseptica PNGase F

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Cat No.

NATE-0601

Description

In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.

Abbr

PNGase F, Native (Elizabethkingia meningoseptica)

Alias

PNGase F

Source

Elizabethkingia meningoseptica

Applications

Used to deglycosylate protein. Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Enzyme Commission Number

EC 3.5.1.52

CAS_No

83534-39-8

Molecular Weight

~36 kDa

Purity

> 95% (SDS-PAGE)

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Optimum pH

~8.6

Storage

2-8°C

Synonyms

glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8

"PNGase F" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1941	PNGase A from Oryza sativa (rice), Recombinant	EC 3.5.1.52		Pichia pastoris	Inquiry
NATE-0600	Native Almonds Glycopeptidase A	EC 3.5.1.52	83534-39-8	Almonds	Inquiry
NATE-0602	Native Elizabethkingia miricola PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia miricola	Inquiry
NATE-0603	Native Flavobacterium meningosepticum PNGase F	EC 3.5.1.52	83534-39-8	Flavobacterium meningosepticum	Inquiry

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