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Native Elizabethkingia meningoseptica PNGase F
Cat No.
NATE-0601
Description
In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.
Abbr
PNGase F, Native (Elizabethkingia meningoseptica)
Alias
PNGase F
Source
Elizabethkingia meningoseptica
Applications
Used to deglycosylate protein. Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.
Enzyme Commission Number
EC 3.5.1.52
CAS No.
83534-39-8
Molecular Weight
~36 kDa
Purity
> 95% (SDS-PAGE)
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
Optimum pH
~8.6
Storage
2-8°C
Synonyms
glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8
Download
Download Datasheet"PNGase F" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1941 | PNGase A from Oryza sativa (rice), Recombinant | EC 3.5.1.52 | Pichia pastoris | Inquiry | |
| NATE-0600 | Native Almonds Glycopeptidase A | EC 3.5.1.52 | 83534-39-8 | Almonds | Inquiry |
| NATE-0602 | Native Elizabethkingia miricola PNGase F | EC 3.5.1.52 | 83534-39-8 | Elizabethkingia miricola | Inquiry |
| NATE-0603 | Native Flavobacterium meningosepticum PNGase F | EC 3.5.1.52 | 83534-39-8 | Flavobacterium meningosepticum | Inquiry |