Native Flavobacterium meningosepticum PNGase F

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Cat No.

NATE-0603

Description

In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.

Abbr

PNGase F, Native (Flavobacterium meningosepticum)

Alias

PNGase F

Source

Flavobacterium meningosepticum

Applications

Glycoprotein analysis Removal of high mannose, hybrid, and complex N-glycans from glycoproteins Free of contaminants (Endo F, proteases, etc.)

Product Overview

PNGase F is purified from Flavobacterium meningosepticum (3) and it is free of proteases and Endo F activities. The following reagents are supplied with this product: Glycoprotein Denaturing Buffer, 10X GlycoBuffer 2, 10X NP-40, 10%

Enzyme Commission Number

EC 3.5.1.52

Activity

500,000 units/ml

CAS_No

83534-39-8

Molecular Weight

36 kDa

Unit Definition

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl (65 NEB units = 1 IUB milliunit).

Stability

Storage Conditions: 20 mM Tris-HCl, 50 mM NaCl, 5 mM Na2EDTA, 50% Glycerol, pH 7.5 25°C Heat Inactivation: 75°C for 10 min

Storage

Store at -20°C.

Synonyms

glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8

"PNGase F" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1941	PNGase A from Oryza sativa (rice), Recombinant	EC 3.5.1.52		Pichia pastoris	Inquiry
NATE-0600	Native Almonds Glycopeptidase A	EC 3.5.1.52	83534-39-8	Almonds	Inquiry
NATE-0601	Native Elizabethkingia meningoseptica PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia meningoseptica	Inquiry
NATE-0602	Native Elizabethkingia miricola PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia miricola	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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