Native Human Carboxypeptidase B

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Cat No.

NATE-0151

Description

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Abbr

CPB1, Native (Human)

Alias

CPB1

Source

Human pancreas

Species

Human

Applications

Carboxypeptidase B from Creative Enzymes has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.

Form

Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3

Enzyme Commission Number

EC 3.4.17.2

Activity

50-55 units/mg protein carboxypeptidase B

CAS_No

9025-24-5

Unit Definition

One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25°C

Storage

−20°C

Pathway

Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem

Function

carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Synonyms

carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5

"CPB1" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0152	Native Porcine Carboxypeptidase B	EC 3.4.17.2	9025-24-5	Porcine pancreas	Inquiry
NATE-0153	Carboxypeptidase-B rat, Recombinant	EC 3.4.17.2	9025-24-5	E. coli	Inquiry
NATE-0993	Carboxypeptidase-B from rat, Recombinant			Pichia pastoris	Inquiry
NATE-1147	Carboxypeptidase B from Porcine, Recombinant	EC 3.4.17.2		Porcine	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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