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Native Porcine NAD(P)H Dehydrogenase (quinone)

Cat No.
NATE-0983
Description
DT Diaphorase is a flavoenzyme that catalyzes the oxidation of reduced di-and triphosphopyridine nucleotides. It contains one mole of FAD per mole of enzyme. The enzyme found in rat liver catalyzes the oxidation of NADH and NADPH by various dyes and quinones. The molecular weight is found to be approximately 48 kDa Da. The pH optimum of the enzyme purified from rat liver is found to be 5.0. It is a cytosolic enzyme that catalyzes the two-electron reduction of various quinones. It catalyzes the conversion of vitamin K to vitamin K hydroquinone for utilization in the post-translational γ-glutamyl carboxylation reactions. These reactions are necessary for several proteins involved in blood coagulation.
Abbr
Diaphorase, Native (Porcine)
Alias
NQO1; QR1; DTD
Source
Porcine heart
Species
Porcine
Applications
Use the diaphorase activity of NAD(P)H Dehydrogenase (quinone) for the determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H, e.g. tetrazolium salts.
Appearance
Yellow suspension in ammonium sulfate, 3.2 mol/l
Product Overview
Dehydrogenase that catalyzes the oxidation of dihydrolipoyl groups and has diaphorase activity. Rely on the proven diagnostic quality of this product.
Activity
>25 U/mg
CAS No.
9032-20-6
Concentration
10±1 mg/ml
pH Stability
5.5-6.5
Stability
At +2 to +8°C within specification range for 12 months.
Synonyms
menadione reductase; phylloquinone reductase; quinone reductase; dehydrogenase, reduced nicotinamide adenine dinucleotide (phosphate, quinone); DT-diaphorase; menadione oxidoreductase; NAD (P)H dehydrogenase; NAD (P)H menadione reductase; NAD (P)H-quinone dehydrogenase; NAD (P)H-quinone oxidoreductase; NAD (P)H: (quinone-acceptor)oxidoreductase

"Diaphorase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1938 Diaphorase 22 from Recombinant E.coli EC 1.8.1.4 9001-18-7 E. coli Inquiry
DIA-320 Diaphorase from E. coli, Recombinant EC 1.8.1.4 9001-18-7 E. coli Inquiry
NATE-1927 Diaphorase-3, recombinant Inquiry
NATE-1901 Native Bacillus stearothermophilus Diaphorase 1 EC 1.6.99.- Bacillus stearothermophilus Inquiry
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