Products

Enzymes for Research, Diagnostic and Industrial Use

Products
Online Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

24 hour
Promise

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

Native Thermoactinomyces intermedius Phenylalanine Dehydrogenase

Cat No.
NATE-1906
Description
Phenylalanine dehydrogenase is a member of a large family of amino-acid dehydrogenases, which includes glutamate dehydrogenase, alanine dehydrogenase, leucine dehydrogenase, lysine €-dehydrogenase, and meso-a,€-diaminopimelate D-dehydrogenase. The three known gene sequences are octomers. It has a two-domain, three-dimensional structure.
Abbr
PheDH, Native (Thermoactinomyces intermedius)
Alias
PheDH
Source
Thermoactinomyces intermedius
Appearance
Ammonium sulphate suspension
Enzyme Commission Number
EC 1.4.1.20
CAS No.
69403-12-9
Contaminants
(as PheDH activity = 100 %)
NADH oxidase: < 0.01 %;
Lactate dehydrogenase: < 0.01 %.
Molecular Weight
ca. 380,000; Subunit molecular weight : ca. 40,000.
pH Stability
5.0 - 10.0
Michaelis Constant
(200 mM Gly-KCl-KOH buffer, pH 11.0, at 30 °C)
L-Phenylalanine: 0.66 mM;
NAD+: 0.05 mM.
Specificity
L-Phenylalanine: 100 %;
L-Tyrosine: 7.6 %;
L-Methionine: 1.5 %.
Unit Definition
One unit of activity is defined as the amount of PheDH that forms 1 μmol of NADH per minute at 30 °C.
Optimum pH
11.5
Thermal stability
No detectable decrease in activity up to 50 °C.
Storage
Stable at 0 to 4 °C for at least six months (Do not freeze).
Synonyms
phenylalanine dehydrogenase; EC 1.4.1.20; L-phenylalanine dehydrogenase; PHD; 69403-12-9
Specific Activity
more than 30 U/mg protein
Reaction
L-Phenylalanine + NAD+ + H2O ←→ Phenylpyruvate + NH4+ + NADH

"PHD" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1803 L-Phenylalanine Dehydrogenase (Crude Enzyme) EC 1.4.1.20 69403-12-9 E. coli Inquiry
NATE-0558 Native Sporosarcina sp. L-Phenylalanine Dehydrogenase EC 1.4.1.20 69403-12-9 Sporosarcina sp. Inquiry
EXWM-1454 phenylalanine dehydrogenase EC 1.4.1.20 69403-12-9 Inquiry
0
Click unfold / close
Inquiry Basket
Delete selected Quote Check Out
Decide to move out of the shopping cart?
Sure No, Back

Please choose product!

< Go Back
You have already added to buy this product