PNGase F from Flavobacterium meningosepticum, Recombinant

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Cat No.

NATE-1287

Description

In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.

Abbr

PNGase F, Recombinant (Flavobacterium meningosepticum)

Alias

PNGase F

Source

E. coli

Species

Flavobacterium meningosepticum

Product Overview

PNGase F, peptide N-glycosidase F, is a recombinantly expressed endoglycosidase from Flavobacterium meningosepticum that cleaves the β-aspartylglucosamine bond between the N-Acetylglucosamine (GlcNAc) and asparagine linkage of N-linked oligosaccharides in glycoproteins. This cleavage point acts as a convenient identification point in subsequent mass spectroscopy analysis.

Form

20 mM Tris - pH 7.5, 50 mM NaCl, 0.5 mM EDTA

Activity

>10 U/mg

CAS_No

83534-39-8

Molecular Weight

34,800 daltons (Apparent)

Purity

>95% by SDS-PAGE

Concentration

7 U/mL

Isoelectric point

8.39

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the release of >95% N-linked oligosaccharides from 60 μmoles of denatured ribonuclease B in 1 hour at 37C, pH 7.5. One micromolar unit of PNGase F activity is equal to 1,000 nanomolar units (IUB milliunits).

Stability

1 year

Storage

2-8°C. Avoid multiple freeze/thaw cycles.

Synonyms

glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8

"PNGase F" Total Products Page

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NATE-1941	PNGase A from Oryza sativa (rice), Recombinant	EC 3.5.1.52		Pichia pastoris	Inquiry
NATE-0600	Native Almonds Glycopeptidase A	EC 3.5.1.52	83534-39-8	Almonds	Inquiry
NATE-0601	Native Elizabethkingia meningoseptica PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia meningoseptica	Inquiry
NATE-0602	Native Elizabethkingia miricola PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia miricola	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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