Pyruvate oxidase preparations without inorganic phosphate

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Cat No.

POPGZ-0001

Description

A microbial pyruvate oxidase preparation supplied without inorganic phosphate. The enzyme catalyzes the oxidative decarboxylation of pyruvate and is suitable for controlled biochemical reaction systems.

Alias

Pyruvate Oxidase, Microorganisms

Source

Microorganisms

Applications

Biochemical research and pyruvate-dependent enzymatic assays.

Appearance

Light yellow to yellow solid

Package

100 U

Product Overview

This solid oxidoreductase preparation provides a specific activity of at least 35 U/mg protein. Its phosphate-free formulation allows the phosphate concentration of the reaction mixture to be adjusted separately.

Form

Solid

Enzyme Commission Number

1.2.3.3

Activity

≥35 U/mg protein

CAS_No

9001-96-1

Specificity

Highest activity toward pyruvate; lower activity has been reported with methylglyoxal and acetaldehyde.

Optimum temperature

38°C initial activity optimum in 50 mM phosphate buffer

Activators

Thiamine pyrophosphate (TPP)
FAD
Divalent cation, such as Mn²⁺

Thermal stability

Activity declines after 10 min of incubation at temperatures above 30°C.

Storage

Store under the conditions specified in the batch certificate of analysis.

Buffer

No inorganic phosphate included

Inhibitors

EDTA, 10 mM: 11% inhibition
KCN, 10 mM: 25% inhibition
No activity change reported with NaN₃ (10 mM), iodoacetamide (1 mM), or p-hydroxymercuribenzoate (1 mM).

Preparation Instructions

The preparation does not contain inorganic phosphate. Supply an appropriate phosphate source through the reaction buffer when carrying out the canonical phosphate-dependent pyruvate oxidase reaction.

Function

Oxidative decarboxylation of pyruvate.

Synonyms

Pyruvate oxidase, microorganisms; PoxB; phosphate-dependent pyruvate oxidase

Substrates

Pyruvate; methylglyoxal; acetaldehyde

Quality

Preparation without inorganic phosphate

Specific Activity

≥35 U/mg protein

Reaction

Pyruvate + phosphate + O₂ → acetyl phosphate + CO₂ + H₂O₂

Notes

The enzyme is phosphate-dependent even though inorganic phosphate is not included in the supplied preparation. No complete standardized assay protocol or unit definition was provided on the source page.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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