Sarcosine Oxidase from E. coli, Recombinant

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Cat No.

DIA-414

Description

Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5, 10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen. sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.

Abbr

SAO, Recombinant (E. coli)

Alias

SAO

Source

E. coli

Species

E. coli

Appearance

Yellow lyophilizate

Enzyme Commission Number

EC 1.5.3.1

Activity

> 15 U/mg lyophilizate

CAS_No

9029-22-5

Contaminants

catalase < 0.5% glucose oxidase < 1.0 x 10^-5%

Molecular Weight

ca. 49 kDa

Isoelectric point

5.3

pH Stability

6.5–10.5

Michaelis Constant

4.7 x 10^-3 M (sarcosine)

Structure

monomer of 43 kDa (SDS-PAGE) one mole of FAD per mole of enzyme

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.7.

Optimum pH

6.7–9.5

Optimum temperature

50°C

Thermal stability

below 55°C

Stability

Stability (liquid form) stable at 37°C for at least two weeks Stability (powder form) stable at 30°C for at least one month

Storage

at -20°C

Stabilizers

Sucrose

Inhibitors

Zn2+, Cu2+, Hg2+, Ag+

Synonyms

Sarcosine Oxidase; EC 1.5.3.1; SAO

"SAO" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-171	Native Microorganism Sarcosine Oxidase	EC 1.5.3.1	9029-22-5	Microorganism	Inquiry
DIA-290	Native E.coli Sarcosine Oxidase		9029-22-5	E. coli	Inquiry
NATE-0664	Native Bacillus sp. Sarcosine Oxidase	EC 1.5.3.1	9029-22-5	Bacillus sp.	Inquiry
EXWM-1537	sarcosine oxidase	EC 1.5.3.1	9029-22-5		Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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