Uricase from E. coli, Recombinant

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Cat No.

DIA-415

Description

The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2

Abbr

UO, Recombinant (E. coli)

Alias

Source

E. coli

Species

E. coli

Appearance

Light brownish lyophilizate

Enzyme Commission Number

EC 1.7.3.3

Activity

> 4 U/mg lyophilizate

CAS_No

9002-12-4

Contaminants

catalase < 1.0%

Molecular Weight

ca. 90 kDa

pH Stability

7.0–11.0

Michaelis Constant

1.1 x 10^-5 M (uric acid)

Structure

2 subunits of 35 kDa (SDS-PAGE)

Unit Definition

One unit (U) is defined as the amount of enzyme which oxidizes 1 μmol of uric acid per min at 25°C and pH 8.5.

Optimum pH

8.5

Optimum temperature

45°C

Thermal stability

below 55°C

Stability

Stability (liquid form) stable at 37°C for at least ten days Stability (powder form) stable at 30°C at least three weeks

Storage

at -20°C

Stabilizers

Citrate, sucrose

Inhibitors

Hg2+, Ag+

Synonyms

urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II

"UO" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-173	Native Bacillus fastidiosus Uricase	EC 1.7.3.3	9002-12-4	Bacillus fastidiosus	Inquiry
DIA-175	Native Candida sp. Uricase	EC 1.7.3.3	9002-12-4	Candida sp.	Inquiry
DIA-276	Native Bacillus sp. Uricase			Bacillus sp.	Inquiry
DIA-294	Uricase, Recombinant	EC 1.7.3.3	9002-12-4		Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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