Recombinant Enzymes for Creatinine Diagnostics: Creatinase, Sarcosine Oxidase & Creatininase

Jul. 31, 2025

At Creative Enzymes, we specialize in supplying researchers and manufacturers with top-grade recombinant enzymes for scientific and industrial applications. Among our wide portfolio, three diagnostic enzymes stand out for their reliability and widespread use: Creatinase (NATE-1241), Sarcosine Oxidase (DIA-414), and Creatininase (NATE-1242) — all expressed recombinantly in Escherichia coli.

These enzymes are critical components in enzymatic assays to measure creatinine and related metabolites, widely applied in in vitro diagnostic (IVD) kits, biochemical research, and automated clinical analyzers. Let's take an in-depth look at their features, parameters, applications, and why Creative Enzymes is your trusted partner for these essential products.

Product Overview and Key Parameters

Product Name	Creatinase from E. coli, Recombinant	Sarcosine Oxidase from E. coli, Recombinant	Creatininase from E. coli, Recombinant
Cat No.	NATE-1241	DIA-414	NATE-1242
CAS No.	37340-58-2	9029-22-5	9025-13-2
EC No.	EC 3.5.3.3	EC 1.5.3.1	EC 3.5.2.10
Source	E. coli	E. coli	E. coli
Activity	≥ 15 U/mg	> 15 U/mg lyophilizate	> 500 U/mg lyophilizate
Appearance	White lyophilizate	Yellow lyophilizate	White lyophilizate
Contaminants	catalase < 0.5%	catalase < 0.5%, glucose oxidase < 1.0 x 10^-5%	catalase < 1.0%
Storage	at -20°C	at -20°C	at -20°C
pH Stability	7.0–9.0	6.5–10.5	7.0–11.0

Why Accurate Creatinine Measurement Matters

Creatinine is a metabolic byproduct of creatine phosphate, a key molecule in muscle energy metabolism. It is produced at a relatively constant rate and cleared from the body almost entirely by the kidneys. An elevated creatinine level is a hallmark indicator of impaired kidney function, making its precise measurement essential for:

Diagnosing and managing chronic kidney disease (CKD)
Monitoring the health of renal transplant patients
Evaluating acute kidney injury (AKI)
Adjusting dosages of drugs that are cleared by the kidneys

Given its clinical importance, the demand for highly reliable and specific creatinine assays is perpetual. While traditional methods like the Jaffe reaction have been used for decades, they are susceptible to interference from other substances in the blood, such as proteins, glucose, and certain drugs, leading to potential inaccuracies. The enzymatic method, employing a cascade of specific enzymes, has emerged as the gold standard for precision and reliability.

The Enzymatic Pathway: A Symphony of Specificity

The three enzymes—Creatininase, Creatinase, and Sarcosine Oxidase—work in a sequential cascade to convert creatinine into a detectable signal, typically hydrogen peroxide (H₂O₂), which can then be measured colorimetrically or fluorometrically.

This multi-step approach ensures that the final signal is directly and specifically proportional to the initial amount of creatinine in the sample, minimizing off-target reactions and enhancing assay accuracy.

Key Advantages of Our Recombinant Diagnostic Enzymes

High Purity & Activity

Each enzyme exceeds >90% purity (SDS-PAGE) with specific activity verified under standardized conditions. This high quality minimizes interference in assays and improves signal-to-noise ratios.

Stable & Convenient

Supplied as lyophilized powders, these enzymes offer extended shelf life at -20°C and maintain activity upon reconstitution. This makes them ideal for use in automated systems and commercial IVD kits.

Proven Recombinant Source

Recombinant production in E. coli ensures scalability and consistent lot-to-lot performance — critical for manufacturers who require predictable enzyme kinetics in kit production.

Comprehensive Support

Our technical team provides detailed product specifications and expert consultation for assay optimization, ensuring seamless integration into existing platforms.

Applications Beyond Clinical Diagnostics

While these enzymes are best known for creatinine determination, their substrate specificities enable diverse uses:

Biochemical research: Studying creatine metabolism, methyl group transfers, or oxidative pathways.
Industrial assays: Monitoring fermentation processes or enzyme performance.
Quality control: Raw material testing or analytical reference methods in manufacturing.

Note: Our Products Cannot Be Used As Medicines Directly For Personal Use.

Why Choose Creative Enzymes?

Deep Expertise in Enzymology

With years of dedicated focus in enzyme R&D, Creative Enzymes is recognized globally for quality and innovation in enzyme production.

Commitment to Quality and Compliance

Our production adheres to rigorous quality control standards. Each batch undergoes multiple analytical checks — ensuring activity, purity, and absence of contaminants.

Customer-Centric Service

We go beyond supply: offering technical support, custom bulk production, and tailored enzyme solutions to fit your specific assay design.

Trusted by Leading Brands

Many diagnostics and biotech companies rely on our enzymes for developing high-performance assay kits, reflecting our reputation and trustworthiness in the field.

Contact Us Today

Ready to elevate your diagnostic assays? Reach out to Creative Enzymes for high-performance recombinant enzymes trusted by researchers worldwide: Contac Us Page.