Native Porcine Aminopeptidase M

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Cat No.

NATE-1912

Description

Native amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.

Source

Porcine kidney

Species

Porcine

Appearance

White crystalline suspension

Enzyme Commission Number

EC 3.4.11.2

Activity

> 50 units/ml

CAS_No

9054-63-1

Contaminants

Oxidases: < 3%

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.

Stability

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.

Storage

2 - 8°C

Synonyms

Aminopeptidase M

Shipping Conditions

Ambient Temperature Only

Formulation

Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.

Specific Activity

> 10 units/mg protein

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Project Description:

For research and industrial use only, not for personal medicinal use.

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