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β-Mannosidase 1A from Pyrococcus furiosus, Recombinant


Cat No.
NATE-1470
Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase 1A, Recombinant (Pyrococcus furiosus)
Source
E. coli
Species
Pyrococcus furiosus
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.25
CAS No.
37288-54-3
Molecular Weight
61.1 kDa
Purity
>90% by SDS-PAGE
Concentration
0.25 mg/mL
Specificity
p-nitrophenyl-β-D-mannopyranoside (ManpbNp), p-nitrophenyl-α-glucopyranoside (GlcpbNp)
Optimum pH
7
Optimum temperature
95 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8
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Related Protocols
b-mannosidase -Enzymatic Assay Protocol
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