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Native Helix pomatia β-Mannosidase


Cat No.
NATE-0778
Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase, Native (Helix pomatia)
Alias
Mannase
Source
Helix pomatia
Form
ammonium sulfate suspension. Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
Enzyme Commission Number
EC 3.2.1.25
Activity
5-30 units/mL
CAS No.
9025-43-8
Unit Definition
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
Storage
2-8°C
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8
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Related Protocols
b-mannosidase -Enzymatic Assay Protocol
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