Products

Enzymes for Research, Diagnostic and Industrial Use

Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase

Cat No.
NATE-0003
Description
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Abbr
3-HBDH, Native (Pseudomonas lemoignei)
Alias
3-HBDH
Source
Pseudomonas lemoignei
Applications
Suitable for the determination of acetoacetate and D (-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Form
Lyophilized powder containing sucrose, β-NAD and Tris buffer salts
Enzyme Commission Number
EC 1.1.1.30
Activity
> 200 units/mg protein
CAS No.
9028-38-0
Unit Definition
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37°C.
Storage
−20°C
Synonyms
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0
Download Datasheet:


“3-HBDH” Total Products Page

Inquiry
Related Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0004
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceRhodopseudomonas sphaeroides
CatalogNATE-0005
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceAlcaligenes faecalis
CatalogNATE-1099
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceProkaryote
Related Services
Related Protocols
ß-HYDROXYBUTYRATE DEHYDROGENASE -Enzymatic Assay Protocol
(S)-DIACETYL REDUCTASE -Enzymatic Assay Protocol
s-diacetyl reductase -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: