Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase

inquiry

Cat No.

DIA-204

Description

In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.

Abbr

D-3-hydroxybutyrate dehydrogenase (Pseudomonas sp.)

Source

Pseudomonas sp.

Applications

This enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.

Appearance

White amorphous powder, lyophilized

Enzyme Commission Number

EC 1.1.1.30

Activity

GradeⅢ 100U/mg-solid or more

CAS_No

9028-38-0

Contaminants

Malate dehydrogenase < 2.0×10⁻³% Lactate dehydrogenase < 2.0×10⁻³% NADH oxidase < 2.0×10⁻³%

Molecular Weight

approx. 130 kDa (by gel filtration)

Isoelectric point

5.6±0.1

pH Stability

pH 5.0-8.5 (25°C, 20hr)

Michaelis Constant

4.2×10⁻⁴M (25°C, pH8.3), 7.0×10⁻⁴M(37°C, pH8.3)(D-3-Hydroxybutyrate) 4.9×10⁻⁵M (25°C, pH8.3), 7.2×10⁻⁵M (37°C, pH8.3)(NAD⁺) 8.1×10⁻⁵M (25°C, pH7.1), 2.4×10⁻⁴M (37°C, pH7.1)(Acetoacetate) 8.4×10⁻⁶M (25°C, pH7.1), 1.5×10⁻⁵M (37°C, pH7.1)(NADH)

Optimum pH

8.3

Optimum temperature

55°C

Thermal stability

below 40°C (pH 6.5, 15min)

Stability

Stable at-20°C for at least one year

Stabilizers

Sucrose, mannitol, bovine serum albumin

Inhibitors

PCMB, MIA, IAA, Ag⁺, Hg⁺⁺, SDS, DAC

Synonyms

(R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30

"3-HBDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1099	Prokaryotic 3-Hydroxybutyrate dehydrogenase, Recombinant	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
NATE-1714	D-3-hydroxybutyrate dehydrogenase from Microorganism	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
NATE-0003	Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase	EC 1.1.1.30	9028-38-0	Pseudomonas lemoignei	Inquiry
NATE-0004	Native Rhodopseudomonas sphaeroides β-Hydroxybutyrate Dehydrogenase	EC 1.1.1.30	9028-38-0	Rhodopseudomonas sphaeroides	Inquiry

+ See More >>

Name:

Email *

Phone:*

Company/Institution:

Country or Region:

Quantity:

Services & Products Interested *

Project Description:

Our Products Cannot Be Used As Medicines Directly For Personal Use.

Products

Online Inquiry