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D-3-hydroxybutyrate dehydrogenase from Microorganism


Cat No.
NATE-1714
Description
In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.
Abbr
HBDH (Microorganism)
Source
Microorganism
Form
Yellowish powder, lyophilized
Enzyme Commission Number
EC 1.1.1.30
Activity
>1500U/mg protein
CAS No.
9028-38-0
Molecular Weight
27.5 kDa (SDS-PAGE)
Isoelectric point
7.25
pH Stability
7.0~10.0 (25℃, 2 hr)
Michaelis Constant
2.1 ×10^-3 M (D-3-Hydroxybutyrate)
Unit Definition
One unit converts one micromole of 3- Hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.
Optimum pH
8
Optimum temperature
6 ℃
Thermal stability
< 37℃(pH 8.5, 30min)
Storage
Store at -20°C.
Inhibitors
Zn2+, Cu2+, Fe3+
Synonyms
(R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30
Notes
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
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Catalog
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CAS No.
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CatalogNATE-1099
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceMicroorganism
CatalogDIA-204
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourcePseudomonas sp.
CatalogNATE-0003
EC No.EC 1.1.1.30
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SourcePseudomonas lemoignei
CatalogNATE-0004
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceRhodopseudomonas sphaeroides
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