Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase

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Cat No.

NATE-0003

Description

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Abbr

3-HBDH, Native (Pseudomonas lemoignei)

Alias

3-HBDH

Source

Pseudomonas lemoignei

Applications

Suitable for the determination of acetoacetate and D (-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Form

Lyophilized powder containing sucrose, β-NAD and Tris buffer salts

Enzyme Commission Number

EC 1.1.1.30

Activity

> 200 units/mg protein

CAS_No

9028-38-0

Unit Definition

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37°C.

Storage

−20°C

Synonyms

3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0

"3-HBDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1099	Prokaryotic 3-Hydroxybutyrate dehydrogenase, Recombinant	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
NATE-1714	D-3-hydroxybutyrate dehydrogenase from Microorganism	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
DIA-204	Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase	EC 1.1.1.30	9028-38-0	Pseudomonas sp.	Inquiry
NATE-0004	Native Rhodopseudomonas sphaeroides β-Hydroxybutyrate Dehydrogenase	EC 1.1.1.30	9028-38-0	Rhodopseudomonas sphaeroides	Inquiry

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