Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and left-handed superhelices.
TOPO I, Recombinant (Human)
Baculovirus-infected insect cells (Spodoptera frugiperda)
Stable for 3 months undiluted
Store at -80°C. It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.
Topoisomerase I; EC 22.214.171.124; type I DNA topoisomerase; untwisting enzyme; relaxing enzyme; nicking-closing enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA topoisomerase; TOPO I