Glycerol-3-Phosphate Dehydrogenase from E. coli, Recombinant

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Cat No.

NATE-1904

Description

α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.

Abbr

G3PDH, Recombinant (E. coli)

Alias

G3PDH; α-GDH

Source

E. coli

Applications

The enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerokinase.

Appearance

Lyophilized

Enzyme Commission Number

EC 1.1.1.8

CAS_No

9075-65-4

Molecular Weight

ca. 73,600; Subunit molecular weight : ca. 36,800.

pH Stability

6.5 - 10.0

Michaelis Constant

(90 mM Bicine buffer pH 9.0, at 37 °C)
Glycerol-3-phosphate: 0.119 mM;
NAD+: 0.036 mM.

Unit Definition

One unit of activity is defined as the amount of G3PDH that forms 1 μmol of NADH per minute at 37 °C.

Optimum pH

Thermal stability

No detectable decrease in activity up to 80 °C.

Storage

Stable at -20 °C for at least one year.

Synonyms

α-glycerol phosphate dehydrogenase (NAD); α-glycerophosphate dehydrogenase (NAD); glycerol 1-phosphate dehydrogenase; glycerol phosphate dehydrogenase (NAD); glycerophosphate dehydrogenase (NAD); hydroglycerophosphate dehydrogenase; L-α-glycerol phosphate dehydrogenase; L-α-glycerophosphate dehydrogenase; L-glycerol phosphate dehydrogenase; L-glycerophosphate dehydrogenase; NAD-α-glycerophosphate dehydrogenase; NAD-dependent glycerol phosphate dehydrogenase; NAD-dependent glycerol-3-phosphate dehydrogenase; NAD-L-glycerol-3-phosphate dehydrogenase; NAD-linked glycerol 3-phosphate dehydrogenase; NADH-dihydroxyacetone phosphate reductase; glycerol-3-phosphate dehydrogenase (NAD); EC 1.1.1.8; 9075-65-4; α-GDH

Specific Activity

more than 7 U/mg protein

Reaction

Glycerol-3-phosphate + NAD+ ←→ Dihydroxyacetone 3-phosphate + NADH+ H+

"GPDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0313	Native Rabbit Glycerol-3-phosphate dehydrogenase	EC 1.1.1.8	9075-65-4	Rabbit Muscle	Inquiry
NATE-0750	Native Rabbit α-Glycerophosphate Dehydrogenase	EC 1.1.1.8	9075-65-4	Rabbit muscle	Inquiry
NATE-0751	Native Rabbit α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase	EC 1.1.1.8	9075-65-4	Rabbit muscle	Inquiry
EXWM-0363	glycerol-3-phosphate dehydrogenase (NAD+)	EC 1.1.1.8	9075-65-4		Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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