Services

Professional and Cost-Saving Solutions

Enzyme Activity Measurement of Glycerol-3-Phosphate Dehydrogenase (NAD+) Using Spectrophotometric Assays



Creative Enzymes provides enzyme activity measurement service to assist scientists’ research in a rapid and accurate way. We are the expert in oxidoreductase activity assays and can offer you reliable assay service with the best equipment, certainly, including the measurement of EC 1.1.1.8, glycerol-3-phosphate dehydrogenase (NAD+).

Glycerol-3-phosphate dehydrogenase (NAD+) is also known as:

Glycerol-3-phosphate dehydrogenase (NAD+) (GPDH; EC 1.1.1.8) catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and L-glycerol-3-phosphate (G3P) using NAD as a cofactor. This enzyme widely exists in mammalians, eukaryotes and prokaryotes, and it participates in glycerophospholipid metabolism. It is important for its direct responsibility of maintaining the NAD/NADH balance in the glycosome by the reoxidation of the NADH produced by glyceraldehyde-3-phosphate dehydrogenase during glycolysis. The generated G3P is then transported across the glycosomal membrane and reoxidized via the G3P: DHAP shuttle that functions in conjunction with a mitochondrial glycerol-3-phosphate oxidase. In yeasts, the enzyme is also involved in the superpathway of phosphatidate biosynthesis. Therefore, GPDH has a promising prospective in pharmaceutical, biotechnical, and nutritional applications.

Enzyme Activity Measurement of Glycerol-3-Phosphate Dehydrogenase (NAD+) Using Spectrophotometric Assays Figure: The crystal structure of Leishmania mexicana glycerol-3-phosphate dehydrogenase in complex with NAD.

Reference: Suresh S. et. al., Structure. 2000 15;8(5):541-52.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. Although other activity assays were reported, the best method to test activity of this enzyme is the spectrophotometric assay through directly detect the absorption of NAD+ at 260nm. Creative Enzymes excels at this type of enzyme activity determination, and we can handle all peculiar situations with targeted programs.

Creative Enzymes have specified experiences in spectrophotometric enzyme activity assays and we stand in a dominant position in this industry. The reason that most customers and scientists chose us is the accurate and proper assay service. Creative enzymes will always be your best partner in your research activities.



Inquiry
Related Products
CatalogNATE-1904
EC No.EC 1.1.1.8
CAS No.9075-65-4
SourceE. coli
CatalogEXWM-0363
EC No.EC 1.1.1.8
CAS No.9075-65-4
Source
CatalogNATE-0313
EC No.EC 1.1.1.8
CAS No.9075-65-4
SourceRabbit Muscle
CatalogNATE-0751
EC No.EC 1.1.1.8
CAS No.9075-65-4
SourceRabbit muscle
CatalogNATE-0750
EC No.EC 1.1.1.8
CAS No.9075-65-4
SourceRabbit muscle
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services of Interest:
Quantity:
Project Description:
Our products are not intended for private therapeutic use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap | Privacy Policy | Terms and Conditions
Copyright ©2011 - 2019 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country or region: