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Enzyme Activity Measurement of Glycerol-3-Phosphate Dehydrogenase (NAD+) Using Spectrophotometric Assays

Creative Enzymes provides enzyme activity measurement service to assist scientists’ research in a rapid and accurate way. We are the expert in oxidoreductase activity assays and can offer you reliable assay service with the best equipment, certainly, including the measurement of EC, glycerol-3-phosphate dehydrogenase (NAD+).

Glycerol-3-phosphate dehydrogenase (NAD+) is also known as:

Glycerol-3-phosphate dehydrogenase (NAD+) (GPDH; EC catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and L-glycerol-3-phosphate (G3P) using NAD as a cofactor. This enzyme widely exists in mammalians, eukaryotes and prokaryotes, and it participates in glycerophospholipid metabolism. It is important for its direct responsibility of maintaining the NAD/NADH balance in the glycosome by the reoxidation of the NADH produced by glyceraldehyde-3-phosphate dehydrogenase during glycolysis. The generated G3P is then transported across the glycosomal membrane and reoxidized via the G3P: DHAP shuttle that functions in conjunction with a mitochondrial glycerol-3-phosphate oxidase. In yeasts, the enzyme is also involved in the superpathway of phosphatidate biosynthesis. Therefore, GPDH has a promising prospective in pharmaceutical, biotechnical, and nutritional applications.

Enzyme Activity Measurement of Glycerol-3-Phosphate Dehydrogenase (NAD+) Using Spectrophotometric Assays Figure: The crystal structure of Leishmania mexicana glycerol-3-phosphate dehydrogenase in complex with NAD.

Reference: Suresh S. et. al., Structure. 2000 15;8(5):541-52.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. Although other activity assays were reported, the best method to test activity of this enzyme is the spectrophotometric assay through directly detect the absorption of NAD+ at 260nm. Creative Enzymes excels at this type of enzyme activity determination, and we can handle all peculiar situations with targeted programs.

Creative Enzymes have specified experiences in spectrophotometric enzyme activity assays and we stand in a dominant position in this industry. The reason that most customers and scientists chose us is the accurate and proper assay service. Creative enzymes will always be your best partner in your research activities.

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