Abbr
Neutral proteinase (Bacillus sp.)
Applications
This enzyme is useful for synthesis of oligopeptides, for proteolysis of insoluble protein and for structural investigation of the protein.
Appearance
White amorphous powder or granule
Enzyme Commission Number
EC 3.4.24.4
Activity
1000U/mg-solid or more
Contaminants
Other proteases <2 X 10⁻²%
Molecular Weight
approx. 25,000 (SDS-PAGE)
pH Stability
pH 6.0-9.0 (40°C,30min)
Unit Definition
One unit causes an increase in the optical density at 275nm corresponding to one microgram of tyrosine per minute under the conditions described below.
Thermal stability
below 70°C (pH 7.5, 10min)
Stability
Stable at 5°C for at least one year
Synonyms
Neutral proteinase; Cathepsin G; Microbial metalloproteinase; EC 3.4.24.4
