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Diaphorase

Official Full Name
Diaphorase
Background
A flavoprotein (FAD). A component of the multienzyme 2-oxo-acid dehydrogenase complexes. In the pyruvate dehydrogenase complex, it binds to the core of EC 2.3.1.12, dihydrolipoyllysine-residue acetyltransferase, and catalyses oxidation of its dihydrolipoyl groups. It plays a similar role in the oxoglutarate and 3-methyl-2-oxobutanoate dehydrogenase complexes. Another substrate is the dihydrolipoyl group in the H-protein of the glycine-cleavage system (click here for diagram), in which it acts, together with EC 1.4.4.2, glycine dehydrogenase (decarboxylating), and EC 2.1.2.10, aminomethyltransferase, to break down glycine. It can also use free dihydrolipoate, dihydrolipoamide or dihydrolipoyllysine as substrate. This enzyme was first shown to catalyse the oxidation of NADH by methylene blue; this activity was called diaphorase. The glycine cleavage system is composed of four components that only loosely associate: the P protein (EC 1.4.4.2), the T protein (EC 2.1.2.10), the L protein (EC 1.8.1.4) and the lipoyl-bearing H protein.
Synonyms
LDP-Glc; LDP-Val; dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; protein-6-N-(dihydrolipoyl)lysine:NAD+ oxidoreductase

Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1901
EC No.EC 1.6.99.-
CAS No.
SourceBacillus stearo...
CatalogEXWM-1648
EC No.EC 1.8.1.4
CAS No.9001-18-7
Source
CatalogEXWM-1601
EC No.EC 1.6.99.3
CAS No.9079-67-8
Source
CatalogEXWM-1600
EC No.EC 1.6.99.1
CAS No.9001-68-7
Source
CatalogNATE-1154
EC No.EC 1.6.99.1
CAS No.9001-68-7
SourceBacillus megate...
CatalogDIA-320
EC No.EC 1.8.1.4
CAS No.
SourceE. coli
CatalogDIA-275
EC No.EC 1.6.99.-
CAS No.
SourceMicroorganism
CatalogNATE-0983
EC No.
CAS No.9032-20-6
SourcePorcine heart
CatalogNATE-0875
EC No.EC 1.8.1.4
CAS No.9001-18-7
SourceClostridium klu...
CatalogNATE-0208
EC No.EC 1.6.99.2
CAS No.
SourceE. coli
CatalogNATE-0209
EC No.EC 1.6.99.2
CAS No.
SourceE. coli
CatalogDIA-187
EC No.EC 1.6.99.-
CAS No.
SourceClostridium sp.
CatalogDIA-142
EC No.EC 1.6.99.3
CAS No.9079-67-8
SourceBacillus megate...
Related Services
Related Protocols
NADPH-FMN OXIDOREDUCTASE -Enzymatic Assay Protocol
cytochrome c reductase -Enzymatic Assay Protocol
DIAPHORASE -Enzymatic Assay Protocol

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