Description
Releases reducing sugars from birchwood xylan (X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. This enzyme does not possess endo-xylanase, arabinoxylanase or β-glucanase activities. β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
Abbr
β-Xylosidase, Recombinant (Bacillus subtilis)
Source
Bacillus subtilis subsp. subtilis str. 168
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.37
Molecular Weight
64909.6 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of oNP per minute from oNP-β-D-xylopyranoside (5 mM) in 50 mM sodium phosphate buffer, pH 7.0, containing 1 mg/mL BSA, at 35°C, and using an extinction coefficient of 18000 M-1cm-1.
Optimum temperature
> 35°C
Storage
Store at 4°C (shipped at room temperature)
Synonyms
β-Xylosidase; β-Xylosidase, thermostable; 9025-53-0
