Description
Chitosanase catalyzes the endohydrolysis of β (1,4) linkages between N-acetyl-D-glucosamine and D-glucosamine residues in partially deacetylated chitosan. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. It is used for the lysis of cell walls of fungi belonging to the group Mucorales. It is found in several types of microorganisms.
Abbr
Chitosanase 18A, Recombinant (Clostridium thermocellum)
Species
Clostridium thermocellum
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.14
Molecular Weight
43.9 kDa
Specificity
Chitin and pNP-chitotriose
Optimum temperature
60 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
Chitosanase; EC 3.2.1.132; 51570-20-8; Chitosan N-acetylglucosaminohydrolase