Products

Enzymes for Research, Diagnostic and Industrial Use

Native Human Carboxypeptidase B


Cat No.
NATE-0151
Description
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Abbr
CPB1, Native (Human)
Alias
CPB1
Source
Human pancreas
Species
Human
Applications
Carboxypeptidase B from Creative Enzymes has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.
Form
Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
Enzyme Commission Number
EC 3.4.17.2
Activity
50-55 units/mg protein carboxypeptidase B
CAS No.
9025-24-5
Unit Definition
One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25°C
Storage
−20°C
Pathway
Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem
Function
carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding
Synonyms
carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5
Download Datasheet:
SDS:


“CPB1” Total Products Page

Inquiry
Related Products
Catalog
Product Name
EC No.
CAS No.
Source
Price
CatalogNATE-0152
EC No.EC 3.4.17.2
CAS No.9025-24-5
SourcePorcine pancreas
CatalogNATE-0153
EC No.EC 3.4.17.2
CAS No.9025-24-5
SourceE. coli
CatalogNATE-0993
EC No.
CAS No.
SourcePichia pastoris
CatalogNATE-1147
EC No.EC 3.4.17.2
CAS No.
SourcePorcine
+ See More >>
Related Protocols
carboxypeptidase_b -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services of Interest:
Quantity:
Project Description:
Our products are not intended for direct clinical use!
*Verification Code:
Verification code

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap | Privacy Policy | Terms and Conditions
Copyright ©2011 - 2021 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country or region:
     
0
Click unfold / close
Inquiry Basket
Delete selected Check Out
Decide to move out of the shopping cart??

Sure No, Back
 

Please choose product!

< Go Back
You have already added to buy this product