Description
EndoS LE is an endoglycosidase for deglycosylation of IgG Fc-glycan moieties. IgGZERO® LE hydrolyzes Fcglycans on IgG of all human IgG subclasses and IgG from the following species: mouse, rat, monkey, sheep, goat, cow and horse. The enzyme has limited activity on high-mannose and hybrid- type glycans.
EndoS LE hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc on the Fc. EndoS LE is a low endotoxin product,use endotoxin free material and solutions.
Physiological reaction conditions at pH 7. 4 and 37°C yields optimal enzyme activity. Other buffers and pH (6-8) are compatible with enzyme activity but the reaction conditions needs to be tested to ensure efficient deglycosylation. EndoS LE has a mass of 110 kDa and contains a His-tag. EndoS LE is for R&D use only.
Abbr
Endo S, Recombinant (Streptocoocus pyogenes)
Species
Streptocoocus pyogenes
Form
Lyophilized in 10 mM sodium phosphate, 150 mM NaCl, pH 7. 4, with no preservatives added.
Purity
> 95% homogeneity as determined by SDS-PAGE analysis.
Unit Definition
One unit deglycosylates > 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.
Storage
It is shipped at ambient temperature. It should be stored at -20°C upon arrival. After reconstitution EndoS LE is stable for 1 month at +4-8°C.
Synonyms
Endoglycosidase S; IgGZERO
Endotoxin Level
< 0.2 EU per vial
