Fructosyl-peptide Oxidase from E. coli, Recombinant

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Cat No.

DIA-410

Description

The fructosyl peptide oxidase is an enzyme that catalyzes the reaction which produces a sugar osone (an α-keto aldehyde), a peptide, and hydrogen peroxide by oxidative cleaving, in the presence of oxygen molecules, the C--N bond in the ketose derivative produced by Amadori rearrangement of glucosylamine produced by the reaction between the hemiacetal of glucose and the N-terminal amino group of a peptide.

Abbr

Fructosyl-peptide Oxidase, Recombinant (E. coli)

Source

E. coli

Species

E. coli

Appearance

Yellow lyophilizate

Enzyme Commission Number

EC 1.5.3

Activity

> 4 U/mg lyophilizate

Molecular Weight

ca. 60 kDa

pH Stability

6.0–9.5

Michaelis Constant

3.4 x 10^-3 M (fructosyl-valyl-histidine) 4.4 x 10^-3 M (fructosyl-glycine) 8.9 x 10^-3 M (Nε-fructosyl-lysine)

Structure

monomer of 52 kDa (SDS-PAGE)

Specificity

fructosyl-valyl-histidine (100), fructosyl-glycine (53), Nε-fructosyl-lysine (84)

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 8.0.

Optimum pH

7.5–8.0

Optimum temperature

35–42°C

Thermal stability

below 45°C

Stability

Stable at 37°C for at least one month

Storage

at -20°C

Stabilizers

EDTA, glutamate

Synonyms

Fructosyl-peptide : oxygen oxidoreductase; EC 1.5.3; Fructosyl-peptide Oxidase

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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