Native Alcaligenes faecalis 3-Hydroxybutyrate Dehydrogenase

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Cat No.

NATE-0005

Description

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Abbr

3-HBDH, Native (Alcaligenes faecalis)

Alias

3-HBDH

Source

Alcaligenes faecalis

Applications

Useful for enzymatic determination of ketone bodies

Appearance

White powder

Product Overview

Is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.

Form

Freeze dried powder

Enzyme Commission Number

EC 1.1.1.30

Activity

> 1,500 U/mg

CAS_No

9028-38-0

Contaminants

NADH oxidase < 0.0004%

Molecular Weight

60±5 kDa (TSK G‒3000SW); 30±5 kDa (SDS‒PAGE)

Isoelectric point

pH 5.0±0.2

pH Stability

5.5-11.0 (37°C, 60 mins)

Michaelis Constant

D‒3‒Hydroxybutyrate 1.6 × 10-3M

Unit Definition

One unit is defined as the amount of enzyme which converts 1 μ mole of 3‒Hydroxybutylate to acetoacetate per minute at 37°C under the conditions specified in the assay procedure.

Optimum pH

8.5

Optimum temperature

45°C (Tris‒HCl buffer)

Thermal stability

Stable at 37°C and below (pH 8.5, 10 mins)

Storage

−20°C

Synonyms

3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0

"3-HBDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1099	Prokaryotic 3-Hydroxybutyrate dehydrogenase, Recombinant	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
NATE-1714	D-3-hydroxybutyrate dehydrogenase from Microorganism	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
DIA-204	Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase	EC 1.1.1.30	9028-38-0	Pseudomonas sp.	Inquiry
NATE-0003	Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase	EC 1.1.1.30	9028-38-0	Pseudomonas lemoignei	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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