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Enzymatic Assay Protocols

Enzymatic Assay Protocols
EC No.
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s-aromatic alcohol dehydrogenase nadp dependent
EC 1.1.1.2 
diacetyl reductase
EC 1.1.1.5 
l-xylulose reductase
EC 1.1.1.10 
GLUCOSE DEHYDROGENASE
EC 1.1.1.47 
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
EC 1.1.1.49 
3α,20β-hydroxysteroid dehydrogenase
EC 1.1.1.53 
L-fucose dehydrogenase
EC 1.1.1.122 
Lactate Oxidase
EC 1.1.3.2 
Glycolate oxidase
EC 1.1.3.15 
GLYCEROL-3-PHOSPHATE OXIDASE
EC 1.1.3.21 
gluconate dehydrogenase nadp independent
EC 1.1.99.3 
FORMATE DEHYDROGENASE
EC 1.2.1.2 
pyruvate oxidase
EC 1.2.3.3 
pyruvate dehydrogenase
EC 1.2.4.1 
acyl coenzyme a oxidase
EC 1.3.3.6 
leucine dehydrogenase
EC 1.4.1.9 
l-glutamate oxidase
EC 1.4.3.11 
CYTOCHROME P450 REDUCTASE
EC 1.6.2.4 
NADH oxidase
EC 1.6.3.3 
nitrate reductase nadph
EC 1.6.6.2 
sulfite oxidase
EC 1.8.3.1 
CYTOCHROME OXIDASE
EC 1.9.3.1 
lipoxidase
EC 1.13.11.12 
DOPAMINE ß-HYDROXYLASE
EC 1.14.17.1 
TYROSINASE Catechol Oxidase Activity
EC 1.14.18.1 
CATECHOL O-METHYL TRANSFERASE
EC 2.1.1.6 
transaldolase
EC 2.2.1.2 
SUCROSE PHOSPHORYLASE
EC 2.4.1.7 
sucrose synthetase
EC 2.4.1.13 
Glutamic Oxalacetic Transaminase
EC 2.6.1.1 
fructose 6 phosphate kinase
EC 2.7.1.11 
gluconate kinase
EC 2.7.1.12 
RIBOKINASE
EC 2.7.1.15 
carbamate kinase
EC 2.7.2.2 
guanylate kinase
EC 2.7.4.8 
polynucleotide phosphorylase
EC 2.7.7.8 
protein kinase c
EC 2.7.11.13 
glycogen synthase kinase 3B
EC 2.7.11.26 
acetylesterase
EC 3.1.1.6 
CHONDRO-4-SULFATASE
EC 3.1.6.9 
DEOXYRIBONUCLEASE II
EC 3.1.22.1 
NUCLEASE P1
EC 3.1.30.1 
isomaltase
EC 3.2.1.10 
DEXTRANASE
EC 3.2.1.11 
α-GLUCOSIDASE
EC 3.2.1.20 
invertase
EC 3.2.1.26 
alfa-L-fucosidase
EC 3.2.1.51 
keratanase
EC 3.2.1.103 
ceramide glycanase
EC 3.2.1.123 
CATHEPSIN C
EC 3.4.14.1 
angiotensin converting enzyme
EC 3.4.15.1 
CARBOXYPEPTIDASE G
EC 3.4.17.11 
a-chymotrypsin
EC 3.4.21.1 
Trypsin TPCK/DITC glass
EC 3.4.21.4 
trypsin insoluble
EC 3.4.21.4 
CATHEPSIN G
EC 3.4.21.20 
ancrod
EC 3.4.21.28 
ELASTASE
EC 3.4.21.36 
Tissue Plasminogen Activator
EC 3.4.21.68 
CATHEPSIN B
EC 3.4.22.1 
CHYMOPAPAIN
EC 3.4.22.6 
RENNIN
EC 3.4.23.4 
CATHEPSIN D
EC 3.4.23.5 
lysostaphin
EC 3.4.24.75 
penicillin amidase
EC 3.5.1.11 
peptidyl arginine deiminase
EC 3.5.3.15 
guanase
EC 3.5.4.3 
creatinine deiminase
EC 3.5.4.21 
INORGANIC PYROPHOSPHATASE
EC 3.6.1.1 
ADENOSINE 5'-TRIPHOSPHATASE
EC 3.6.1.3 
a-acetolactate decarboxylase
EC 4.1.1.5 
apotryptophanase
EC 4.1.99.1 
tryptophanase
EC 4.1.99.1 
crotonase
EC 4.2.1.17 
PECTIN LYASE
EC 4.2.2.10 
chondroitinase c
EC 4.2.2 
l-aspartase
EC 4.3.1.1 
L-methionine gamma-lyase
EC 4.4.1.11 
D-Ribulose 5-phosphate 3-epimerase
EC 5.1.3.1 
Peptidyl-Prolyl Isomerase
EC 5.2.1.8 
PROTEIN DISULFIDE ISOMERASE
EC 5.3.4.1 
aminoacyl-trna synthetase
EC 6.1.1 
ENDONUCLEASE
 
MICROPEROXIDASE
 
peptidase
 
PROTEASE INHIBITOR, of Calcium Activated Neutral Protease
 
Protease
 
BROMELAIN INHIBITOR
 
CUCUMISIN
 
CYTOHELICASE
 
Carboxypeptidase inhibitor
 
D-AMINO ACID OXIDASE APOENZYME
 
L-FUCOSE DEHYDROGENASE
EC 1.1.1.122 
GLYOXYLATE REDUCTASE
EC 1.1.1.26 
L-LACTIC DEHYDROGENASE
EC 1.1.1.27 
(R)-AROMATIC ALCOHOL DEHYDROGENASE,NADP+ Dependent
EC 1.1.1.2 
s-diacetyl reductase
EC 1.1.1.304 
malic enzyme
EC 1.1.1.40 
isocitric dehydrogenase
EC 1.1.1.42 
ANNITOL DEHYDROGENASE
EC 1.1.1.67 
glycerol dehydrogenase
EC 1.1.1.6 
ß-GALACTOSE_DEHYDROGENASE
EC 1.1.1.48 
3-PHOSPHOGLYCERATE DEHYDROGENASE
EC 1.1.1.95 
xanthine oxidase
EC 1.1.3.22 
CHOLESTEROL OXIDASE
EC 1.1.3.6 
d-fructose dehydrogenase
EC 1.1.99.11 
GLUCONATE DEHYDROGENASE, NAD(P) Independent
EC 1.1.99.3 
PEROXIDASE from Soybean
EC 1.11.1.7 
MYELOPEROXIDASE
EC 1.11.1.7 
salicylate hydroxylase
EC 1.14.13.1 
TYROSINASE Polyphenol Oxidase Activity
EC 1.14.18.1 
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
EC 1.2.1.12 
FORMATE DEHYDROGENASE
EC 1.2.1.2 
FORMALDEHYDE DEHYDROGENASE
EC 1.2.1.46 
BILIRUBIN OXIDASE
EC 1.3.3.5 
acyl coenzyme a oxidase
EC 1.3.3.6 
L-PHENYLALANINE DEHYDROGENASE
EC 1.4.1.20 
L-GLUTAMIC DEHYDROGENASE
EC 1.4.1.3 
d-amino acid oxidase
EC 1.4.3.3 
TYRAMINE OXIDASE
EC 1.4.3.4 
SACCHAROPINE DEHYDROGENASE,NAD+,Lysine Forming
EC 1.5.1.7 
NADPH-FMN OXIDOREDUCTASE
EC 1.6.99.1 
cytochrome c reductase
EC 1.6.99.3 
DIHYDROPTERIDINE REDUCTASE
EC 1.6.99.7 
URICASE
EC 1.7.3.3 
DIAPHORASE
EC 1.8.1.4 
NITRATE REDUCTASE(CYTOCHROME)
EC 1.9.6.1 
CATECHOL-O-METHYL TRANSFERASE
EC 2.1.1.6 
ORNITHINE CARBAMYL TRANSFERASE
EC 2.1.3.3 
TRANSKETOLASE
EC 2.2.1.1 
choline acetyltransferase
EC 2.3.1.6 
transglutaminase
EC 2.3.2.13 
glycogen synthase
EC 2.4.1.11 
Glycogen Branching Enzyme
EC 2.4.1.18 
GLUTATHIONE-S-TRANSFERA 1-Chloro-2,4-Dinitrobenzene as SubstrateSE
EC 2.5.1.18 
GLUTATHIONE-S-TRANSFERA p-Nitrobenzyl Chloride as SubstrateSE
EC 2.5.1.18 
glutamic pyruvic transaminase
EC 2.6.1.2 
RIBOKINASE
EC 2.7.1.15 
HEXOKINASE
EC 2.7.1.1 
nad-kinase
EC 2.7.1.23 
CHOLINE KINASE
EC 2.7.1.32 
PYRUVATE KINASE
EC 2.7.1.40 
GALACTOKINASE
EC 2.7.1.6 
FRUCTOSE-6-PHOSPHATE KINASE,PYROPHOSPHATE DEPENDENT from Mung Bean
EC 2.7.1.90 
PROTEIN KINASE C
EC 2.7.11.13 
PROTEIN KINASE
EC 2.7.11.1 
acetate kinase from Bacillus stearothermophilus
EC 2.7.2.1 
CREATINE PHOSPHOKINASE
EC 2.7.3.2 
arginine kinase
EC 2.7.3.3 
PHOSPHORIBOSYL-PYROPHOSPHATE SYNTHETASE
EC 2.7.6.1 
GALACTOSE-1-PHOSPHATE URIDYL TRANSFERASE
EC 2.7.7.12 
RHODANESE
EC 2.8.1.1 
SUCCINYL COENZYME A TRANSFERASE
EC 2.8.3.5 
CHOLESTEROL ESTERASE
EC 3.1.1.13 
lipoprotein lipase
EC 3.1.1.34 
LIPASE
EC 3.1.1.3 
PHOSPHOLIPASE A2
EC 3.1.1.4 
cholinesterase-acetyl
EC 3.1.1.7 
CHOLINESTERASE,BUTYRYL
EC 3.1.1.8 
RIBONUCLEASE T2
EC 3.1.27.1 
D-FRUCTOSE-1,6-DIPHOSPHATASE
EC 3.1.3.11 
PHYTASE
EC 3.1.3.26 
phosphodieterase
EC 3.1.4.1 
GLYCEROPHOSPHORYLCHOLINE PHOSPHODIESTERASE
EC 3.1.4.2 
PHOSPHOLIPASE C
EC 3.1.4.3 
PHOSPHOLIPASE D
EC 3.1.4.4 
CHONDRO-4-SULFATASE
EC 3.1.6.9 
dextranase
EC 3.2.1.11 
pectolyase
EC 3.2.1.15,EC_4.2.2.10 
neuraminidase
EC 3.2.1.18 
MALTASE
EC 3.2.1.20 
β-GALACTOSIDASE
EC 3.2.1.2 
HYALURONIDASE
EC 3.2.1.35 
HESPERIDINASE
EC 3.2.1.40 
PULLULANASE
EC 3.2.1.41 
a-N-ACETYLGALACTOSAMINIDASE
EC 3.2.1.49 
LAMINARINASE
EC 3.2.1.6 
XYLANASE Activity in DRISELASE
EC 3.2.1.8 
S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE
EC 3.3.1.1 
ALANINE AMINOPEPTIDASE
EC 3.4.11.14 
PROLINE IMINOPEPTIDASE
EC 3.4.11.5 
proline iminopeptidase
EC 3.4.11.5 
PROLIDASE
EC 3.4.13.9 
carboxypeptidase y
EC 3.4.16.1 
carboxypeptidase w
EC 3.4.16.1 
CARBOXYPEPTIDASE P
EC 3.4.17.16 
L-pyroglutamate aminopeptidase
EC 3.4.19.3 
pyroglutamate aminopeptidase
EC 3.4.19.3 
a-CHYMOTRYPSIN
EC 3.4.21.1 
KALLIKREIN from Human Plasma
EC 3.4.21.34 
protease
EC 3.4.21.40 
PROTEASE(Endoproteinase Lys-C)
EC 3.4.21.50 
CATHEPSIN B
EC 3.4.22.1 
PAPAIN, INSOLUBLE
EC 3.4.22.2 
papain or ficin on agarose
EC 3.4.22.2 
BROMELAIN
EC 3.4.22.32 
CATHEPSIN D
EC 3.4.23.5 
proteinase A
EC 3.4.23.6 
LYSOSTAPHIN
EC 3.4.24.75 
renin
EC 3.4.99.19 
ARYL ACYLAMIDASE
EC 3.5.1.13 
GLUTAMINASE(From E
EC 3.5.1.2 
glutaminase
EC 3.5.1.2 
AMIDASE
EC 3.5.1.4 
CREATININASE From Pseudomonas species
EC 3.5.2.10 
allantoinase
EC 3.5.2.5 
PENICILLINASE Cephaloridine as Substrate
EC 3.5.2.6 
PENICILLINASE
EC 3.5.2.6 
d-hydantoinase
EC 3.5.22 
ARGINASE
EC 3.5.3.1 
CREATINASE
EC 3.5.3.3 
APYRASE AMP as Substrate
EC 3.6.1.5 
pyrophosphatase nucleotide
EC 3.6.1.9 
L-glutamic decarboxylase
EC 4.1.1.15 
L-LYSINE DECARBOXYLASE
EC 4.1.1.18 
PHOSPHO(ENOL)PYRUVATE CARBOXYLASE
EC 4.1.1.31 
ISOCITRATE LYASE
EC 4.1.3.1 
N-ACETYLNEURAMINIC ACID ALDOLASE
EC 4.1.3.3 
citrate lyase
EC 4.1.3.6 
CITRATE SYNTHASE
EC 4.1.3.7 
d-AMINOLEVULINATE DEHYDRATASE
EC 4.2.1.24 
ACONITASE
EC 4.2.1.3 
CHONDROITINASE B
EC 4.2.2.19 
heparinase III
EC 4.2.2.8 
HISTIDASE
EC 4.3.1.3 
ARGININOSUCCINATE LYASE
EC 4.3.2.1 
uridine 5-diphosphogalactose 4-epimerase
EC 5.1.3.2 
Peptidyl-Prolyl Isomerase
EC 5.2.1.8 
phosphoriboisomerase
EC 5.3.1.6 
PHOSPHOMANNOSE ISOMERASE
EC 5.3.1.8 
phosphoglucose isomerase
EC 5.3.1.9 
AMINOACYL-tRNA SYNTHETASE
EC 6.1.1 
ACYL-ACYL CARRIER PROTEIN SYNTHETASE
EC 6.2.1.20 
SUCCINIC THIOKINASE
EC 6.2.1.4 
GLUTAMINE SYNTHETASE
EC 6.3.1.2 
GABase
 
GLYCOGEN SYNTHASE KINASE 3B
 
HEPARINASE II
 
Hemicellulase
 
Hirudin
 
METALLOENDOPEPTIDASE
 
MUTANOLYSIN
 
NARINGINASE
 
Orotidine-5'-phosphate pyrophosphopylase & orotidine-5'-phosphate decarboxylase
 
PEPSINOGEN
 
PHOSPHODIESTERASE,3'5'-CYCLIC NUCLEOTIDE ACTIVATOR DEFICIENT
 
PHOSPHODIESTERASE,3'5'-CYCLIC NUCLEOTIDE Crude Complex
 
PHOSPHODIESTERASE
 
PROTEASE1 Casein as a Substrate
 
THROMBOCYTIN
 
a-CHYMOTRYPSINOGEN A
 
actomyosin ATPase
 
alpha-1-antitrypsin
 
a-KETOGLUTARATE-DEHYDROGENASE
 
CALCINEURIN
 
CERULOPLASMIN
 
polyol-dehydrogenase
EC 1.1.1.14 
MYO-INOSITOL-DEHYDROGENASE
EC 1.1.1.18 
URIDINE-5'-DIPHOSPHOGLUCOSE DEHYDROGENASE
EC 1.1.1.22 
d-lactic_dehydrogenase
EC 1.1.1.28 
(S)-DIACETYL REDUCTASE
EC 1.1.1.304 
ß-HYDROXYBUTYRATE DEHYDROGENASE
EC 1.1.1.30 
ß-HYDROXYACYL-COA DEHYDROGENASE
EC 1.1.1.35 
6-PHOSPHOGLUCONIC DEHYDROGENASE
EC 1.1.1.44 
ß-HYDROXYSTEROID DEHYDROGENASE
EC 1.1.1.51 
a,20ß-HYDROXYSTEROID-DEHYDROGENASE
EC 1.1.1.533 
a-GLYCEROPHOSPHATE DEHYDROGENASE
EC 1.1.1.8 
galactose_oxidase
EC 1.1.3.9 
phenolase
EC 1.10.3.1 
ascorbate-oxidase
EC 1.10.3.3 
chloroperoxidase
EC 1.11.1.10 
NADH-peroxidase
EC 1.11.1.1 
protocatechuate_3,4-dioxygenase
EC 1.13.11.3 
p-hydroxybenzoate_hydroxylase
EC 1.14.13.2 
tyrosine_hydroxylase
EC 1.14.16.2 
glyceralde-3-phosphate dehydrogenase
EC 1.2.1.12 
succinic semialdehyde dehydrogenase
EC 1.2.1.16 
formaldehyde_dehydrogenase
EC 1.2.1.1 
aldehyde_dehydrogenase
EC 1.2.1.5 
PYRUVATE DEHYDROGENASE
EC 1.2.4.1 
OROTATE REDUCTASE
EC 1.3.1.14 
L-alanine_dehydrogenase
EC 1.4.1.1 
L-glutamic dehydrogenase (β-nadp)
EC 1.4.1.4 
lysine-oxidase
EC 1.4.3.14 
L-amino_acid_oxidase
EC 1.4.3.2 
PLASMA-AMINE-OXIDASE
EC 1.4.3.6 
OCTOPINE-DEHYDROGENASE
EC 1.5.1.11 
5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE
EC 1.5.1.5 
SARCOSINE-OXIDASE
EC 1.5.3.1 
sarcosine dehydrogenase
EC 1.5.99.1 
GLUTATHIONE-REDUCTASE-Coenzyme-A-Glutathione-Reductase-Activity1
EC 1.6.4.2 
THIOREDOXIN REDUCTASE
EC 1.6.4.5 
NITRATE-REDUCTASE
EC 1.6.6.1 
PHENYLETHANOLAMINE-N-METHYLTRANSFERASE
EC 2.1.1.28 
FORMIMINO-L-GLUTAMIC-ACID-TRANSFERASE
EC 2.1.2.5 
aspartate_transcarbamylase
EC 2.1.3.2 
KANAMYCIN 6'-ACETYLTRANSFERASE
EC 2.3.1.55 
carnitine_acetyltransferase
EC 2.3.1.7 
SUCROSE-PHOSPHATE-SYNTHETASE
EC 2.4.1.14 
URIDINE-5'-DIPHOSPHOGLUCURONYL-TRANSFERASE
EC 2.4.1.17 
GALACTOSYLTRANSFERASE
EC 2.4.1.22 
dextran sucrase
EC 2.4.1.5 
thymidine phosphorylase
EC 2.4.2.4 
HYPOXANTHINE-GUANINE PHOSPHORIBOSYL TRANSFERASE
EC 2.4.2.8 
sialyltransferase
EC 2.4.99.1 
phosphoribulokinase
EC 2.7.1.19 
glucokinase
EC 2.7.1.2 
PHOSPHORYLASE-KINASE
EC 2.7.1.38 
casein_kinase_ii
EC 2.7.11.1 
NUCLEOSIDE MONOPHOSPHATE KINASE
EC 2.7.4.4 
ADENOSINE 5'-TRIPHOSPHATE SULFURYLASE
EC 2.7.7.4 
ribonucleic acid polymerase
EC 2.7.7.6 
URIDINE-5'-DIPHOSPHOGLUCOSE-PYROPHOSPHORYLASE
EC 2.7.7.9 
pectinesterase
EC 3.1.1.11 
esterase
EC 3.1.1.1 
GLYOXALASE II
EC 3.1.2.6 
ribonuclease_u1
EC 3.1.27.3 
ribonuclease_u2
EC 3.1.27.4 
PHOSPHATASE, ALKALINE
EC 3.1.3.1 
phosphatase,acid,prostatic
EC 3.1.3.2 
3'-nucleotidase
EC 3.1.3.6 
PHOSPHOLIPASE-C- PHOSPHATIDYLINOSITOL-SPECIFIC
EC 3.1.4.10 
SPHINGOMYELINASE
EC 3.1.4.12 
sulfatase
EC 3.1.6.1 
CHITINASE
EC 3.2.1.14 
polygalacturonase
EC 3.2.1.15 
ß-GLUCOSIDASE
EC 3.2.1.21 
a-galactosidase
EC 3.2.1.22 
ß-GALACTOSIDASE
EC 3.2.1.23 
a-mannosidase
EC 3.2.1.24 
b-mannosidase
EC 3.2.1.25 
trehalase
EC 3.2.1.28 
ß-N-ACETYLGLUCOSAMINIDASE
EC 3.2.1.30 
b-n-acetylglucosaminidase
EC 3.2.1.52 
mycodextranase
EC 3.2.1.61 
AGARASE
EC 3.2.1.81 
ADP-RIBOSYLCYCLASE
EC 3.2.2.6 
thioglucosidase
EC 3.2.3.1 
aminopeptidase
EC 3.4.11.10 
arylamidase
EC 3.4.11.2 
leukotriene_d4_hydrolase
EC 3.4.13.19 
prolidase
EC 3.4.13.9 
dipeptidyl_peptidase
EC 3.4.14.5 
carboxypeptidase_b
EC 3.4.17.2 
TRYPSIN-TPCKDITC-GLASS
EC 3.4.21.4 
urokinase
EC 3.4.21.73 
plasmin
EC 3.4.21.7 
enterokinase
EC 3.4.21.9 
pepsin insoluble
EC 3.4.23.1 
collagenase
EC 3.4.24.3 
penicillin amidase
EC 3.5.1.11 
aryl_acylamidase
EC 3.5.1.13 
ACYLASE I
EC 3.5.1.14 
asparaginase
EC 3.5.1.1 
UREASE (ATP-Hydrolyzing)
EC 3.5.1.45 
urease
EC 3.5.1.5 
D-hydantoinase
EC 3.5.2.2 
adenosine-deaminase
EC 3.5.4.4 
5'-ADENYLIC ACID DEAMINASE
EC 3.5.4.6 
5'-ADENYLIC-ACID-DEAMINASE
EC 3.5.4.6 
L-ORNITHINE DECARBOXYLASE
EC 4.1.1.17 
L-arginine_decarboxylase
EC 4.1.1.19 
pyruvate_decarboxylase
EC 4.1.1.1 
OROTIDINE-5'-MONOPHOSPHATE DECARBOXYLASE
EC 4.1.1.23 
L-TYROSINE-DECARBOXYLASE
EC 4.1.1.25 
OXALATE-DECARBOXYLASE
EC 4.1.1.2 
D-RIBULOSE-1,5-DIPHOSPHATE-CARBOXYLASE
EC 4.1.1.39 
oxalacetate decarboxylase
EC 4.1.1.3 
L-PHENYLALANINE DECARBOXYLASE
EC 4.1.1.53 
mandelonitrile lyase
EC 4.1.2.10 
aldolase
EC 4.1.2.13 
malate synthase
EC 4.1.3.2 
enolase
EC 4.2.1.11 
CHONDROITINASE B
EC 4.2.2.19 
chondroitinase_abc
EC 4.2.2.4 
chondroitinase_ac
EC 4.2.2.5 
heparinase1
EC 4.2.2.7 
phenylalanine_ammonia-lyase
EC 4.3.1.5 
adenylosuccinate_lyase
EC 4.3.2.2 
glyoxalase_i
EC 4.4.1.5 
FK-BINDING PROTEIN Peptidyl-Prolyl Isomerase
EC 5.2.1.8 
PHOSPHOGLYCERATE MUTASE
EC 5.4.2.1 
acyl_coenzyme_a_synthetase
EC 6.2.1.3 
ß-NAD SYNTHETASE
EC 6.3.5.1 
ß-NAD-SYNTHETASE
EC 6.3.5.1 
pyruvate_carboxylase
EC 6.4.1.1 
ELASTIN-CONGO RED
 
GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE-3-PHOSPHOGLYCERIC-PHOSPHOKINASE
 
HEPARINASE II
 
LAR-PROTEIN TYROSINE PHOSPHATASE
 
peroxidase_insoluble
 
ribonuclease_c
 
Determination of Activity for Superoxide Dismutase (SOD)
EC 1.15.1.1