Enzymatic Assay Protocols

EC No.

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s-aromatic alcohol dehydrogenase nadp dependent

EC 1.1.1.2

diacetyl reductase

EC 1.1.1.5

l-xylulose reductase

EC 1.1.1.10

GLUCOSE DEHYDROGENASE

EC 1.1.1.47

GLUCOSE-6-PHOSPHATE DEHYDROGENASE

EC 1.1.1.49

3α,20β-hydroxysteroid dehydrogenase

EC 1.1.1.53

L-fucose dehydrogenase

EC 1.1.1.122

Lactate Oxidase

EC 1.1.3.2

Glycolate oxidase

EC 1.1.3.15

GLYCEROL-3-PHOSPHATE OXIDASE

EC 1.1.3.21

gluconate dehydrogenase nadp independent

EC 1.1.99.3

FORMATE DEHYDROGENASE

EC 1.2.1.2

pyruvate oxidase

EC 1.2.3.3

pyruvate dehydrogenase

EC 1.2.4.1

acyl coenzyme a oxidase

EC 1.3.3.6

leucine dehydrogenase

EC 1.4.1.9

l-glutamate oxidase

EC 1.4.3.11

CYTOCHROME P450 REDUCTASE

EC 1.6.2.4

NADH oxidase

EC 1.6.3.3

nitrate reductase nadph

EC 1.6.6.2

sulfite oxidase

EC 1.8.3.1

CYTOCHROME OXIDASE

EC 1.9.3.1

lipoxidase

EC 1.13.11.12

DOPAMINE ß-HYDROXYLASE

EC 1.14.17.1

TYROSINASE Catechol Oxidase Activity

EC 1.14.18.1

CATECHOL O-METHYL TRANSFERASE

EC 2.1.1.6

transaldolase

EC 2.2.1.2

SUCROSE PHOSPHORYLASE

EC 2.4.1.7

sucrose synthetase

EC 2.4.1.13

Glutamic Oxalacetic Transaminase

EC 2.6.1.1

fructose 6 phosphate kinase

EC 2.7.1.11

gluconate kinase

EC 2.7.1.12

RIBOKINASE

EC 2.7.1.15

carbamate kinase

EC 2.7.2.2

guanylate kinase

EC 2.7.4.8

polynucleotide phosphorylase

EC 2.7.7.8

protein kinase c

EC 2.7.11.13

glycogen synthase kinase 3B

EC 2.7.11.26

acetylesterase

EC 3.1.1.6

CHONDRO-4-SULFATASE

EC 3.1.6.9

DEOXYRIBONUCLEASE II

EC 3.1.22.1

NUCLEASE P1

EC 3.1.30.1

isomaltase

EC 3.2.1.10

DEXTRANASE

EC 3.2.1.11

α-GLUCOSIDASE

EC 3.2.1.20

invertase

EC 3.2.1.26

alfa-L-fucosidase

EC 3.2.1.51

keratanase

EC 3.2.1.103

ceramide glycanase

EC 3.2.1.123

CATHEPSIN C

EC 3.4.14.1

angiotensin converting enzyme

EC 3.4.15.1

CARBOXYPEPTIDASE G

EC 3.4.17.11

a-chymotrypsin

EC 3.4.21.1

Trypsin TPCK/DITC glass

EC 3.4.21.4

trypsin insoluble

EC 3.4.21.4

CATHEPSIN G

EC 3.4.21.20

ancrod

EC 3.4.21.28

ELASTASE

EC 3.4.21.36

Tissue Plasminogen Activator

EC 3.4.21.68

CATHEPSIN B

EC 3.4.22.1

CHYMOPAPAIN

EC 3.4.22.6

RENNIN

EC 3.4.23.4

CATHEPSIN D

EC 3.4.23.5

lysostaphin

EC 3.4.24.75

penicillin amidase

EC 3.5.1.11

peptidyl arginine deiminase

EC 3.5.3.15

guanase

EC 3.5.4.3

creatinine deiminase

EC 3.5.4.21

INORGANIC PYROPHOSPHATASE

EC 3.6.1.1

ADENOSINE 5'-TRIPHOSPHATASE

EC 3.6.1.3

a-acetolactate decarboxylase

EC 4.1.1.5

apotryptophanase

EC 4.1.99.1

tryptophanase

EC 4.1.99.1

crotonase

EC 4.2.1.17

PECTIN LYASE

EC 4.2.2.10

chondroitinase c

EC 4.2.2

l-aspartase

EC 4.3.1.1

L-methionine gamma-lyase

EC 4.4.1.11

D-Ribulose 5-phosphate 3-epimerase

EC 5.1.3.1

Peptidyl-Prolyl Isomerase

EC 5.2.1.8

PROTEIN DISULFIDE ISOMERASE

EC 5.3.4.1

aminoacyl-trna synthetase

EC 6.1.1

ENDONUCLEASE

MICROPEROXIDASE

peptidase

PROTEASE INHIBITOR, of Calcium Activated Neutral Protease

Protease

BROMELAIN INHIBITOR

CUCUMISIN

CYTOHELICASE

Carboxypeptidase inhibitor

D-AMINO ACID OXIDASE APOENZYME

L-FUCOSE DEHYDROGENASE

EC 1.1.1.122

GLYOXYLATE REDUCTASE

EC 1.1.1.26

L-LACTIC DEHYDROGENASE

EC 1.1.1.27

(R)-AROMATIC ALCOHOL DEHYDROGENASE,NADP+ Dependent

EC 1.1.1.2

s-diacetyl reductase

EC 1.1.1.304

malic enzyme

EC 1.1.1.40

isocitric dehydrogenase

EC 1.1.1.42

ANNITOL DEHYDROGENASE

EC 1.1.1.67

glycerol dehydrogenase

EC 1.1.1.6

ß-GALACTOSE_DEHYDROGENASE

EC 1.1.1.48

3-PHOSPHOGLYCERATE DEHYDROGENASE

EC 1.1.1.95

xanthine oxidase

EC 1.1.3.22

CHOLESTEROL OXIDASE

EC 1.1.3.6

d-fructose dehydrogenase

EC 1.1.99.11

GLUCONATE DEHYDROGENASE, NAD(P) Independent

EC 1.1.99.3

PEROXIDASE from Soybean

EC 1.11.1.7

MYELOPEROXIDASE

EC 1.11.1.7

salicylate hydroxylase

EC 1.14.13.1

TYROSINASE Polyphenol Oxidase Activity

EC 1.14.18.1

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

EC 1.2.1.12

FORMATE DEHYDROGENASE

EC 1.2.1.2

FORMALDEHYDE DEHYDROGENASE

EC 1.2.1.46

BILIRUBIN OXIDASE

EC 1.3.3.5

acyl coenzyme a oxidase

EC 1.3.3.6

L-PHENYLALANINE DEHYDROGENASE

EC 1.4.1.20

L-GLUTAMIC DEHYDROGENASE

EC 1.4.1.3

d-amino acid oxidase

EC 1.4.3.3

TYRAMINE OXIDASE

EC 1.4.3.4

SACCHAROPINE DEHYDROGENASE,NAD+,Lysine Forming

EC 1.5.1.7

NADPH-FMN OXIDOREDUCTASE

EC 1.6.99.1

cytochrome c reductase

EC 1.6.99.3

DIHYDROPTERIDINE REDUCTASE

EC 1.6.99.7

URICASE

EC 1.7.3.3

DIAPHORASE

EC 1.8.1.4

NITRATE REDUCTASE(CYTOCHROME)

EC 1.9.6.1

CATECHOL-O-METHYL TRANSFERASE

EC 2.1.1.6

ORNITHINE CARBAMYL TRANSFERASE

EC 2.1.3.3

TRANSKETOLASE

EC 2.2.1.1

choline acetyltransferase

EC 2.3.1.6

transglutaminase

EC 2.3.2.13

glycogen synthase

EC 2.4.1.11

Glycogen Branching Enzyme

EC 2.4.1.18

GLUTATHIONE-S-TRANSFERA 1-Chloro-2,4-Dinitrobenzene as SubstrateSE

EC 2.5.1.18

GLUTATHIONE-S-TRANSFERA p-Nitrobenzyl Chloride as SubstrateSE

EC 2.5.1.18

glutamic pyruvic transaminase

EC 2.6.1.2

RIBOKINASE

EC 2.7.1.15

HEXOKINASE

EC 2.7.1.1

nad-kinase

EC 2.7.1.23

CHOLINE KINASE

EC 2.7.1.32

PYRUVATE KINASE

EC 2.7.1.40

GALACTOKINASE

EC 2.7.1.6

FRUCTOSE-6-PHOSPHATE KINASE,PYROPHOSPHATE DEPENDENT from Mung Bean

EC 2.7.1.90

PROTEIN KINASE C

EC 2.7.11.13

PROTEIN KINASE

EC 2.7.11.1

acetate kinase from Bacillus stearothermophilus

EC 2.7.2.1

CREATINE PHOSPHOKINASE

EC 2.7.3.2

arginine kinase

EC 2.7.3.3

PHOSPHORIBOSYL-PYROPHOSPHATE SYNTHETASE

EC 2.7.6.1

GALACTOSE-1-PHOSPHATE URIDYL TRANSFERASE

EC 2.7.7.12

RHODANESE

EC 2.8.1.1

SUCCINYL COENZYME A TRANSFERASE

EC 2.8.3.5

CHOLESTEROL ESTERASE

EC 3.1.1.13

lipoprotein lipase

EC 3.1.1.34

LIPASE

EC 3.1.1.3

PHOSPHOLIPASE A2

EC 3.1.1.4

cholinesterase-acetyl

EC 3.1.1.7

CHOLINESTERASE,BUTYRYL

EC 3.1.1.8

RIBONUCLEASE T2

EC 3.1.27.1

D-FRUCTOSE-1,6-DIPHOSPHATASE

EC 3.1.3.11

PHYTASE

EC 3.1.3.26

phosphodieterase

EC 3.1.4.1

GLYCEROPHOSPHORYLCHOLINE PHOSPHODIESTERASE

EC 3.1.4.2

PHOSPHOLIPASE C

EC 3.1.4.3

PHOSPHOLIPASE D

EC 3.1.4.4

CHONDRO-4-SULFATASE

EC 3.1.6.9

dextranase

EC 3.2.1.11

pectolyase

EC 3.2.1.15，EC_4.2.2.10

neuraminidase

EC 3.2.1.18

MALTASE

EC 3.2.1.20

β-GALACTOSIDASE

EC 3.2.1.2

HYALURONIDASE

EC 3.2.1.35

HESPERIDINASE

EC 3.2.1.40

PULLULANASE

EC 3.2.1.41

a-N-ACETYLGALACTOSAMINIDASE

EC 3.2.1.49

LAMINARINASE

EC 3.2.1.6

XYLANASE Activity in DRISELASE

EC 3.2.1.8

S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE

EC 3.3.1.1

ALANINE AMINOPEPTIDASE

EC 3.4.11.14

PROLINE IMINOPEPTIDASE

EC 3.4.11.5

proline iminopeptidase

EC 3.4.11.5

PROLIDASE

EC 3.4.13.9

carboxypeptidase y

EC 3.4.16.1

carboxypeptidase w

EC 3.4.16.1

CARBOXYPEPTIDASE P

EC 3.4.17.16

L-pyroglutamate aminopeptidase

EC 3.4.19.3

pyroglutamate aminopeptidase

EC 3.4.19.3

a-CHYMOTRYPSIN

EC 3.4.21.1

KALLIKREIN from Human Plasma

EC 3.4.21.34

protease

EC 3.4.21.40

PROTEASE(Endoproteinase Lys-C)

EC 3.4.21.50

CATHEPSIN B

EC 3.4.22.1

PAPAIN, INSOLUBLE

EC 3.4.22.2

papain or ficin on agarose

EC 3.4.22.2

BROMELAIN

EC 3.4.22.32

CATHEPSIN D

EC 3.4.23.5

proteinase A

EC 3.4.23.6

LYSOSTAPHIN

EC 3.4.24.75

renin

EC 3.4.99.19

ARYL ACYLAMIDASE

EC 3.5.1.13

GLUTAMINASE(From E

EC 3.5.1.2

glutaminase

EC 3.5.1.2

AMIDASE

EC 3.5.1.4

CREATININASE From Pseudomonas species

EC 3.5.2.10

allantoinase

EC 3.5.2.5

PENICILLINASE Cephaloridine as Substrate

EC 3.5.2.6

PENICILLINASE

EC 3.5.2.6

d-hydantoinase

EC 3.5.22

ARGINASE

EC 3.5.3.1

CREATINASE

EC 3.5.3.3

APYRASE AMP as Substrate

EC 3.6.1.5

pyrophosphatase nucleotide

EC 3.6.1.9

L-glutamic decarboxylase

EC 4.1.1.15

L-LYSINE DECARBOXYLASE

EC 4.1.1.18

PHOSPHO(ENOL)PYRUVATE CARBOXYLASE

EC 4.1.1.31

ISOCITRATE LYASE

EC 4.1.3.1

N-ACETYLNEURAMINIC ACID ALDOLASE

EC 4.1.3.3

citrate lyase

EC 4.1.3.6

CITRATE SYNTHASE

EC 4.1.3.7

d-AMINOLEVULINATE DEHYDRATASE

EC 4.2.1.24

ACONITASE

EC 4.2.1.3

CHONDROITINASE B

EC 4.2.2.19

heparinase III

EC 4.2.2.8

HISTIDASE

EC 4.3.1.3

ARGININOSUCCINATE LYASE

EC 4.3.2.1

uridine 5-diphosphogalactose 4-epimerase

EC 5.1.3.2

Peptidyl-Prolyl Isomerase

EC 5.2.1.8

phosphoriboisomerase

EC 5.3.1.6

PHOSPHOMANNOSE ISOMERASE

EC 5.3.1.8

phosphoglucose isomerase

EC 5.3.1.9

AMINOACYL-tRNA SYNTHETASE

EC 6.1.1

ACYL-ACYL CARRIER PROTEIN SYNTHETASE

EC 6.2.1.20

SUCCINIC THIOKINASE

EC 6.2.1.4

GLUTAMINE SYNTHETASE

EC 6.3.1.2

GABase

GLYCOGEN SYNTHASE KINASE 3B

HEPARINASE II

Hemicellulase

Hirudin

METALLOENDOPEPTIDASE

MUTANOLYSIN

NARINGINASE

Orotidine-5'-phosphate pyrophosphopylase & orotidine-5'-phosphate decarboxylase

PEPSINOGEN

PHOSPHODIESTERASE,3'5'-CYCLIC NUCLEOTIDE ACTIVATOR DEFICIENT

PHOSPHODIESTERASE,3'5'-CYCLIC NUCLEOTIDE Crude Complex

PHOSPHODIESTERASE

PROTEASE1 Casein as a Substrate

THROMBOCYTIN

a-CHYMOTRYPSINOGEN A

actomyosin ATPase

alpha-1-antitrypsin

a-KETOGLUTARATE-DEHYDROGENASE

CALCINEURIN

CERULOPLASMIN

polyol-dehydrogenase

EC 1.1.1.14

MYO-INOSITOL-DEHYDROGENASE

EC 1.1.1.18

URIDINE-5'-DIPHOSPHOGLUCOSE DEHYDROGENASE

EC 1.1.1.22

d-lactic_dehydrogenase

EC 1.1.1.28

(S)-DIACETYL REDUCTASE

EC 1.1.1.304

ß-HYDROXYBUTYRATE DEHYDROGENASE

EC 1.1.1.30

ß-HYDROXYACYL-COA DEHYDROGENASE

EC 1.1.1.35

6-PHOSPHOGLUCONIC DEHYDROGENASE

EC 1.1.1.44

ß-HYDROXYSTEROID DEHYDROGENASE

EC 1.1.1.51

a,20ß-HYDROXYSTEROID-DEHYDROGENASE

EC 1.1.1.533

a-GLYCEROPHOSPHATE DEHYDROGENASE

EC 1.1.1.8

galactose_oxidase

EC 1.1.3.9

phenolase

EC 1.10.3.1

ascorbate-oxidase

EC 1.10.3.3

chloroperoxidase

EC 1.11.1.10

NADH-peroxidase

EC 1.11.1.1

protocatechuate_3,4-dioxygenase

EC 1.13.11.3

p-hydroxybenzoate_hydroxylase

EC 1.14.13.2

tyrosine_hydroxylase

EC 1.14.16.2

glyceralde-3-phosphate dehydrogenase

EC 1.2.1.12

succinic semialdehyde dehydrogenase

EC 1.2.1.16

formaldehyde_dehydrogenase

EC 1.2.1.1

aldehyde_dehydrogenase

EC 1.2.1.5

PYRUVATE DEHYDROGENASE

EC 1.2.4.1

OROTATE REDUCTASE

EC 1.3.1.14

L-alanine_dehydrogenase

EC 1.4.1.1

L-glutamic dehydrogenase (β-nadp)

EC 1.4.1.4

lysine-oxidase

EC 1.4.3.14

L-amino_acid_oxidase

EC 1.4.3.2

PLASMA-AMINE-OXIDASE

EC 1.4.3.6

OCTOPINE-DEHYDROGENASE

EC 1.5.1.11

5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE

EC 1.5.1.5

SARCOSINE-OXIDASE

EC 1.5.3.1

sarcosine dehydrogenase

EC 1.5.99.1

GLUTATHIONE-REDUCTASE-Coenzyme-A-Glutathione-Reductase-Activity1

EC 1.6.4.2

THIOREDOXIN REDUCTASE

EC 1.6.4.5

NITRATE-REDUCTASE

EC 1.6.6.1

PHENYLETHANOLAMINE-N-METHYLTRANSFERASE

EC 2.1.1.28

FORMIMINO-L-GLUTAMIC-ACID-TRANSFERASE

EC 2.1.2.5

aspartate_transcarbamylase

EC 2.1.3.2

KANAMYCIN 6'-ACETYLTRANSFERASE

EC 2.3.1.55

carnitine_acetyltransferase

EC 2.3.1.7

SUCROSE-PHOSPHATE-SYNTHETASE

EC 2.4.1.14

URIDINE-5'-DIPHOSPHOGLUCURONYL-TRANSFERASE

EC 2.4.1.17

GALACTOSYLTRANSFERASE

EC 2.4.1.22

dextran sucrase

EC 2.4.1.5

thymidine phosphorylase

EC 2.4.2.4

HYPOXANTHINE-GUANINE PHOSPHORIBOSYL TRANSFERASE

EC 2.4.2.8

sialyltransferase

EC 2.4.99.1

phosphoribulokinase

EC 2.7.1.19

glucokinase

EC 2.7.1.2

PHOSPHORYLASE-KINASE

EC 2.7.1.38

casein_kinase_ii

EC 2.7.11.1

NUCLEOSIDE MONOPHOSPHATE KINASE

EC 2.7.4.4

ADENOSINE 5'-TRIPHOSPHATE SULFURYLASE

EC 2.7.7.4

ribonucleic acid polymerase

EC 2.7.7.6

URIDINE-5'-DIPHOSPHOGLUCOSE-PYROPHOSPHORYLASE

EC 2.7.7.9

pectinesterase

EC 3.1.1.11

esterase

EC 3.1.1.1

GLYOXALASE II

EC 3.1.2.6

ribonuclease_u1

EC 3.1.27.3

ribonuclease_u2

EC 3.1.27.4

PHOSPHATASE, ALKALINE

EC 3.1.3.1

phosphatase,acid,prostatic

EC 3.1.3.2

3'-nucleotidase

EC 3.1.3.6

PHOSPHOLIPASE-C- PHOSPHATIDYLINOSITOL-SPECIFIC

EC 3.1.4.10

SPHINGOMYELINASE

EC 3.1.4.12

sulfatase

EC 3.1.6.1

CHITINASE

EC 3.2.1.14

polygalacturonase

EC 3.2.1.15

ß-GLUCOSIDASE

EC 3.2.1.21

a-galactosidase

EC 3.2.1.22

ß-GALACTOSIDASE

EC 3.2.1.23

a-mannosidase

EC 3.2.1.24

b-mannosidase

EC 3.2.1.25

trehalase

EC 3.2.1.28

ß-N-ACETYLGLUCOSAMINIDASE

EC 3.2.1.30

b-n-acetylglucosaminidase

EC 3.2.1.52

mycodextranase

EC 3.2.1.61

AGARASE

EC 3.2.1.81

ADP-RIBOSYLCYCLASE

EC 3.2.2.6

thioglucosidase

EC 3.2.3.1

aminopeptidase

EC 3.4.11.10

arylamidase

EC 3.4.11.2

leukotriene_d4_hydrolase

EC 3.4.13.19

prolidase

EC 3.4.13.9

dipeptidyl_peptidase

EC 3.4.14.5

carboxypeptidase_b

EC 3.4.17.2

TRYPSIN-TPCKDITC-GLASS

EC 3.4.21.4

urokinase

EC 3.4.21.73

plasmin

EC 3.4.21.7

enterokinase

EC 3.4.21.9

pepsin insoluble

EC 3.4.23.1

collagenase

EC 3.4.24.3

penicillin amidase

EC 3.5.1.11

aryl_acylamidase

EC 3.5.1.13

ACYLASE I

EC 3.5.1.14

asparaginase

EC 3.5.1.1

UREASE (ATP-Hydrolyzing)

EC 3.5.1.45

urease

EC 3.5.1.5

D-hydantoinase

EC 3.5.2.2

adenosine-deaminase

EC 3.5.4.4

5'-ADENYLIC ACID DEAMINASE

EC 3.5.4.6

5'-ADENYLIC-ACID-DEAMINASE

EC 3.5.4.6

L-ORNITHINE DECARBOXYLASE

EC 4.1.1.17

L-arginine_decarboxylase

EC 4.1.1.19

pyruvate_decarboxylase

EC 4.1.1.1

OROTIDINE-5'-MONOPHOSPHATE DECARBOXYLASE

EC 4.1.1.23

L-TYROSINE-DECARBOXYLASE

EC 4.1.1.25

OXALATE-DECARBOXYLASE

EC 4.1.1.2

D-RIBULOSE-1,5-DIPHOSPHATE-CARBOXYLASE

EC 4.1.1.39

oxalacetate decarboxylase

EC 4.1.1.3

L-PHENYLALANINE DECARBOXYLASE

EC 4.1.1.53

mandelonitrile lyase

EC 4.1.2.10

aldolase

EC 4.1.2.13

malate synthase

EC 4.1.3.2

enolase

EC 4.2.1.11

CHONDROITINASE B

EC 4.2.2.19

chondroitinase_abc

EC 4.2.2.4

chondroitinase_ac

EC 4.2.2.5

heparinase1

EC 4.2.2.7

phenylalanine_ammonia-lyase

EC 4.3.1.5

adenylosuccinate_lyase

EC 4.3.2.2

glyoxalase_i

EC 4.4.1.5

FK-BINDING PROTEIN Peptidyl-Prolyl Isomerase

EC 5.2.1.8

PHOSPHOGLYCERATE MUTASE

EC 5.4.2.1

acyl_coenzyme_a_synthetase

EC 6.2.1.3

ß-NAD SYNTHETASE

EC 6.3.5.1

ß-NAD-SYNTHETASE

EC 6.3.5.1

pyruvate_carboxylase

EC 6.4.1.1

ELASTIN-CONGO RED

GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE-3-PHOSPHOGLYCERIC-PHOSPHOKINASE

HEPARINASE II

LAR-PROTEIN TYROSINE PHOSPHATASE

peroxidase_insoluble

ribonuclease_c

Determination of Activity for Superoxide Dismutase (SOD)

EC 1.15.1.1