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Native Clostridium perfringens (C. welchii) Phospholipase C

Cat No.
NATE-0593
Description
Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Abbr
PLC, Native (Clostridium perfringens (C. welchii))
Alias
PLC
Source
Clostridium perfringens (C. welchii)
Form
Type I, Lyophilized powder in buffered salts; Type II, lyophilized powder; Type III, buffered aqueous glycerol solution; Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA; Type IV, lyophilized powder, Contains phosphate buffer salts, EDTA and stabilizer.
Enzyme Commission Number
EC 3.1.4.3
Activity
Type I, > 150 units/mg protein; Type II, 10-50 units/mg protein; Type III, Type IV, > 1,000 units/mg protein (Lowry).
CAS No.
9001-86-9
Unit Definition
One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37°C.
Storage
−20°C
Warnings
Chromatographically purified
Synonyms
Phospholipase C; PLC; 9001-86-9; lipophosphodiesterase I; lecithinase C; Clostridium welchii α-toxin; Clostridium oedematiens β-and γ-toxins; lipophosphodiesterase C; phosphatidase C; heat-labile hemolysin; α-toxin; EC 3.1.4.3
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0592
EC No.EC 3.1.4.3
CAS No.9001-86-9
SourceBacillus cereus
Realted Service
Realted Protocols
PHOSPHOLIPASE C -Enzymatic Assay Protocol
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