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β-Galactose Dehydrogenase S from Pseudomonas fluorescens, Recombinant

Cat No.
NATE-1710
Description
In enzymology, a galactose 1-dehydrogenase (EC 1.1.1.48) is an enzyme that catalyzes the chemical reaction: D-galactose + NAD+ rightleftharpoons D-galactono-1,4-lactone + NADH + H+. Thus, the two substrates of this enzyme are D-galactose and NAD+, whereas its 3 products are D-galactono-1,4-lactone, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in galactose metabolism.
Abbr
β-Galactose dehydrogenase, Recombinant (P. fluorescens)
Source
E. coli
Species
Pseudomonas fluorescens
Applications
β-Galactose Dehydrogenase S has been used in the colorimetric microassay method to determine the level of galactose and galactose-1-phosphate in blood.
Form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.
Enzyme Commission Number
EC 1.1.1.48
Activity
80 U/mg protein
Contaminants
<0.01% ADH, <0.01% ß-galactosidase, <0.1% LDH, <0.05% NADH oxidase
Storage
Store at -20°C.
Synonyms
D-galactose:NAD+ 1-oxidoreductase; D-galactose dehydrogenase; beta-galactose dehydrogenase; NAD+-dependent D-galactose dehydrogenase; galactose 1-dehydrogenase; EC 1.1.1.48; Galactose dehydrogenase
Notes
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
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Related Protocols
ß-GALACTOSE_DEHYDROGENASE -Enzymatic Assay Protocol
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