Description
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN).
Abbr
GLA, Recombinant (Cellvibrio mixtus)
Source
Cellvibrio mixtus ATCC 12120
Enzyme Commission Number
EC 3.2.1.22
Molecular Weight
44582.3 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1 μmol of p-nitrophenol per minute from p-nitrophenyl-α-D-Dgalactopyranoside (1 mM in the assay) in 25 mM Tris-HCl buffer, Ph 8.5, at 37°C.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Dissolve to 1 mg/mL in 25 mM Tris-HCl, pH 8.5, 20 mM NaCl and aliquot for storage at -20°C. The enzyme should be stable for 6 months when stored in this manner. α-Galactosidase 27A (GH27), in the lyophilized form, will remain stable up to 3 years if stored as specified.
Synonyms
Alpha-Galactosidase; Galactosidase; EC 3.2.1.22; GLA; GALA; melibiase; α-D-galactosidase; α-galactosidase A; α-galactoside galactohydrolase
