α-Galactosidase from Cellvibrio mixtus, Recombinant

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Cat No.

NATE-1175

Description

Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN).

Abbr

GLA, Recombinant (Cellvibrio mixtus)

Alias

GLA

Source

Cellvibrio mixtus ATCC 12120

Form

Lyophilised powder

Enzyme Commission Number

EC 3.2.1.22

Activity

150 U/mg

CAS_No

9025-35-8

Molecular Weight

44582.3 Da

Purity

> 95 % as judged by SDS-PAGE

Unit Definition

One unit is defined as the amount of enzyme required to release 1 μmol of p-nitrophenol per minute from p-nitrophenyl-α-D-Dgalactopyranoside (1 mM in the assay) in 25 mM Tris-HCl buffer, Ph 8.5, at 37°C.

Optimum pH

8.5

Optimum temperature

37°C

Storage

Store at 4°C (shipped at room temperature)

Preparation Instructions

Dissolve to 1 mg/mL in 25 mM Tris-HCl, pH 8.5, 20 mM NaCl and aliquot for storage at -20°C. The enzyme should be stable for 6 months when stored in this manner. α-Galactosidase 27A (GH27), in the lyophilized form, will remain stable up to 3 years if stored as specified.

Synonyms

Alpha-Galactosidase; Galactosidase; EC 3.2.1.22; GLA; GALA; melibiase; α-D-galactosidase; α-galactosidase A; α-galactoside galactohydrolase

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