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α-Galactosidase from Cellvibrio mixtus, Recombinant

Cat No.
NATE-1175
Description
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN).
Abbr
GLA, Recombinant (Cellvibrio mixtus)
Alias
GLA
Source
Cellvibrio mixtus ATCC 12120
Form
Lyophilised powder
Enzyme Commission Number
EC 3.2.1.22
Activity
150 U/mg
CAS No.
9025-35-8
Molecular Weight
44582.3 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1 μmol of p-nitrophenol per minute from p-nitrophenyl-α-D-Dgalactopyranoside (1 mM in the assay) in 25 mM Tris-HCl buffer, Ph 8.5, at 37°C.
Optimum pH
8.5
Optimum temperature
37°C
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Dissolve to 1 mg/mL in 25 mM Tris-HCl, pH 8.5, 20 mM NaCl and aliquot for storage at -20°C. The enzyme should be stable for 6 months when stored in this manner. α-Galactosidase 27A (GH27), in the lyophilized form, will remain stable up to 3 years if stored as specified.
Synonyms
Alpha-Galactosidase; Galactosidase; EC 3.2.1.22; GLA; GALA; melibiase; α-D-galactosidase; α-galactosidase A; α-galactoside galactohydrolase
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