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β-Glucosidase from Clostridium thermocellum, Recombinant


Cat No.
NATE-1182
Description
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Abbr
β-Glucosidase, Recombinant (Clostridium thermocellum)
Source
Clostridium thermocellum DSM 1237
Form
Supplied in 35 mM HEPES buffer, pH 7.8, containing 750 mM NaCl, 5 mM imidazole, 3.5 mM CaCl2, 0.02 % (w/v) sodium azide and 25 % (v/v) glycerol.
Enzyme Commission Number
EC 3.2.1.21
Activity
33 U/mg
CAS No.
9001-42-7
Molecular Weight
52700 Da
Purity
> 95 % as judged by SDS-PAGE
Concentration
20 U/mL
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of p-nitrophenol per minute from p-nitrophenyl-β-Dglucopyranoside (1 mM in the assay) in 50 mM phosphate buffer, pH 6.0, at 60°C.
Optimum pH
6 (stable from 5.5 - 7)
Optimum temperature
60°C (stable up to 65°C)
Storage
Store at -20°C (shipped at room temperature)
Synonyms
EC 3.2.1.21; gentiobiase; cellobiase; emulsin; elaterase; aryl-beta-glucosidase; beta-D-glucosidase; beta-glucoside glucohydrolase; arbutinase; amygdalinase; p-nitrophenyl beta-glucosidase; primeverosidase; amygdalase; linamarase; salicilinase; beta-1,6-glucosidase
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Related Protocols
ß-GLUCOSIDASE -Enzymatic Assay Protocol
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