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β-Glucosidase from Rhizobium etli, Recombinant


Cat No.
NATE-1183
Description
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Abbr
β-Glucosidase, Recombinant (Rhizobium etli)
Source
Rhizobium etli CFN 42
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.21
Activity
159.5 U/mg
CAS No.
9001-42-7
Molecular Weight
53741.8 Da
Purity
> 95 % as judged by SDS-PAGE
Concentration
315.9 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of pNP per minute from pNP-β-D-glucopyranoside (2 mM) in 100 mM sodium acetate buffer, pH 5.4, at 40°C, and using an extinction coefficient of 18000 M-1cm-1.
Optimum pH
5.4
Storage
Store at 4°C (shipped at room temperature)
Synonyms
EC 3.2.1.21; gentiobiase; cellobiase; emulsin; elaterase; aryl-beta-glucosidase; beta-D-glucosidase; beta-glucoside glucohydrolase; arbutinase; amygdalinase; p-nitrophenyl beta-glucosidase; primeverosidase; amygdalase; linamarase; salicilinase; beta-1,6-glucosidase
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Related Protocols
ß-GLUCOSIDASE -Enzymatic Assay Protocol
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