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3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA 24-hydroxylase

Cat No.
EXWM-1103
Description
Requires ATP. The reaction in mammals possibly involves dehydrogenation to give a 24(25)-double bond followed by hydration. However, in amphibians such as the Oriental fire-bellied toad (Bombina orientalis), it is probable that the product is formed via direct hydroxylation of the saturated side chain of (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate and not via hydration of a 24(25) double bond. In microsomes, the free acid is preferred to the coenzyme A ester, whereas in mitochondria, the coenzyme A ester is preferred to the free-acid form of the substrate.
Form
Liquid or lyophilized powder
Enzyme Commission Number
CAS No.
119799-47-2
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
Synonyms
trihydroxycoprostanoyl-CoA oxidase; THC-CoA oxidase; THCA-CoA oxidase; 3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA oxidase; 3α,7α,12α-trihydroxy-5β-cholestan-26-oate 24-hydroxylase
Reaction
(25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-CoA + H2O + acceptor = (24R,25R)-3α,7α,12α,24-tetrahydroxy-5β-cholestan-26-oyl-CoA + reduced acceptor
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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