Description
The catalytic subunit of cAMP-dependent Protein Kinase (PKA) is a serine/threonine protein kinase, which combines, in the absence of cAMP, with the regulatory subunit to form the inactive PKA holoenzyme. Since this is the free catalytic subunit alone, no cAMP is required for activation.
Form
50 mM NaCl, 20 mM Tris-HCl (pH 7.5 at 25°C), 1 mM Na2 EDTA, 2 mM DTT and 50% glycerol.
Enzyme Commission Number
EC 2.7.11.11
Concentration
2,500,000 units/ml
Unit Definition
One unit is defined as the amount of PKA catalytic subunit required to catalyze the transfer of 1 pmol of phosphate to Kemptide, LRRASLG (100 µM) in 1 minute at 30°C in a total reaction volume of 25μL.
Synonyms
Protein Kinase A catalytic subunit; Protein kinase A; PKA; PKAC; cAMP-dependent protein kinase catalytic subunit; PRKAC
Specific Activity
5,000,000 units/mg
