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Casein Kinase Iδ rat, Recombinant


Cat No.
NATE-0140
Description
The Casein kinase 1 family of protein kinases are serine/threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. CK1 isoforms are involved in Wnt signaling, circadian rhythms, nucleo-cytoplasmic shuttling of transcription factors, DNA repair, and DNA transcription.
Abbr
CK-Iδ, Recombinant (Rat)
Alias
CK-Iδ; CK-I
Source
E. coli
Species
Rat
Product Overview
C-terminal truncated, N-terminal His-tag/S-tag, > 90% (SDS-PAGE), recombinant, expressed in E. coli, solution
Form
solution
Activity
1,000-3,000 units/mg protein
Molecular Weight
mol wt 41.8 kDa
Purity
> 90% (SDS-PAGE)
Unit Definition
One unit catalyzes the transfer of 1 nmol of phosphate from ATP into α-casein per minute at 30°C at pH 7.5.
Stability
−20°C
Buffer
Solution in 20 mM HEPES pH 7.5, 1 mM DTT, 1 mM EDTA, 0.1% CHAPS, 250 mM NaCl, 20 μg/ml aprotinin, and 50% glycerol.
Preparation Instructions
CK-IδΔ317 is a truncated form of CK-Iδ comprising the amino acids 1-317 of the N-terminal catalytic domain.
Pathway
Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; Circadian rhythm-mammal, organism-specific biosystem; Circadian rhythm-mammal, conserved biosystem; G2/M Transition, organism-specific biosystem; Gap junction, organism-specific biosystem
Function
ATP binding; ATP binding; glycoprotein binding; nucleotide binding; peptide binding; phosphoprotein binding; protein binding; protein kinase activity; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity, transferring phosphorus-containing groups
Synonyms
Casein Kinase Iδ; Casein Kinase I; CK-Iδ; CK-I; Non-specific serine/threonine protein kinase; Protein phosphokinase; Protein serine kinase; Protein serine-threonine kinase; Protein-serine kinase; Serine kinase; Serine protein kinase; Serine (threonine) protein kinase; Serine-specific protein kinase; Serine/threonine protein kinase; Threonine-specific protein kinase
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