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DNA Polymerase Alpha from Human, Recombinant

Cat No.
NATE-1922
Description
DNA polymerase alpha also known as Pol α is an enzyme complex found in eukaryotes that is involved in initiation of DNA replication. The DNA polymerase alpha complex consists of 4 subunits: POLA1, POLA2, PRIM1, and PRIM2.
Species
Human
Enzyme Commission Number
EC 2.7.7.7
Activity
16,835 units/mg, 5 units/µl
CAS No.
9012-90-2
Concentration
0.297 mg/ml
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmol of total nucleotide into acid-insoluble form in 60 minutes at 37ºC.
Storage
Store at -80°C
Note: Avoid multiple freeze / thaw cycles or frequent temperature changes
Buffer
20 mM Tris-HCl (pH 8.0)
0.25 mM EDTA
50 mM NaCl
1.0 mM β-mercaptoethanol
0.1% Triton X-100™
50% (v/v) glycerol
Synonyms
DNA polymerase alpha; DNA polymerase; Pol α
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