Description
DNA polymerase alpha also known as Pol α is an enzyme complex found in eukaryotes that is involved in initiation of DNA replication. The DNA polymerase alpha complex consists of 4 subunits: POLA1, POLA2, PRIM1, and PRIM2.
Enzyme Commission Number
EC 2.7.7.7
Activity
16,835 units/mg, 5 units/µl
Concentration
0.297 mg/ml
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmol of total nucleotide into acid-insoluble form in 60 minutes at 37ºC.
Storage
Store at -80°C
Note: Avoid multiple freeze / thaw cycles or frequent temperature changes
Buffer
20 mM Tris-HCl (pH 8.0)
0.25 mM EDTA
50 mM NaCl
1.0 mM β-mercaptoethanol
0.1% Triton X-100™
50% (v/v) glycerol
Synonyms
DNA polymerase alpha; DNA polymerase; Pol α
