Description
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.
Species
Flavobacterium heparinum
Enzyme Commission Number
EC 4.2.2.7
Specificity
Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate).
Unit Definition
One international unit (IU) of recombinant heparinase I is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 35 °C and pH 7.0.
Stability
Expiration of heparinase I is 12 months from manufacturing date, frozen at -96 to -20 °C in PBS.
Synonyms
Heparinase; Heparin lyase; Heparin eliminase; Heparin-sulfate lyase; Heparin-sulfate eliminase; Heparitin-sulfate lyase; Heparinase I
