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Immobilized Ficin (Agarose Resin)

Cat No.
NATE-1865
Description
Immobilized Ficin specifically cleaves mouse IgG1 into F(ab´)2 or Fab fragments in the presence of 4mM or 25mM cysteine, respectively. Fragment generation from other species and isotypes may also be possible through modification of the cysteine concentration and other digestion parameters.
Ficin, isolated from fig latex, is a sulfhydryl protease with a similar active site to bromelain and papain; however, yields and immunoreactivity are better when digesting mouse IgG1 with ficin. Ficin acts on bonds involving uncharged and aromatic amino acids. Ficin (25K) is effective at pH 4-9.5 with an optimum pH of 6.5.
Ficin immobilization enhances stability against denaturation, heat and autolysis. Immobilization also eliminates the potential for antibody-enzyme adducts that result in continued sample digestion. Additionally, immobilization allows ficin to be reused.
Form
33% slurry (i.e., 5mL of settled resin is equal to 15mL of slurry)
Enzyme Commission Number
EC 3.4.22.3
Activity
~1.2mg/mL of settled resin
Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Synonyms
Immobilized Ficin
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0255
EC No.EC 3.4.22.3
CAS No.9001-33-6
SourceFig tree latex
CatalogEXWM-4204
ProductNameficain
EC No.EC 3.4.22.3
CAS No.9001-33-6
Source
Realted Protocols
BROMELAIN -Enzymatic Assay Protocol
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