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Immobilized human antithrombin
Cat No.
NATE-1756
Description
May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7. 4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7. 4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7. 4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7. 4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.
Source
Human plasma
Species
Human
Form
Frozen liquid
Molecular Weight
58200
Purity
>95% by SDS-PAGE analysis
Concentration
1. 0 mg/ml
Stability
12 months from delivery
Storage
4°C
Buffer
0.1M Tris-HCl; 0.15M NaCl; 0.02% Sodium Azide; pH 7. 4
Preservative
Sodium Azide
Synonyms
Antithrombin; AT; ATIII; AT3, AT3D, THPH7, serpin family C member 1
Extinction coefficient
0.65
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