Description
Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase. Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.
Abbr
Alanine Racemase, Native (Bacillus stearothermophilus)
Source
Bacillus stearothermophilus
Applications
Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Creative Enzymes, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D-[14C]alanine.
Form
lyophilized powder. Lyophilized from 50 mM phosphate buffer, pH 7.5
Enzyme Commission Number
EC 5.1.1.1
Activity
> 10 Iunits/mg solid
Molecular Weight
Mr 78 kDa (2 subunits 39 kDa each)
Unit Definition
One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30°C in a coupled assay system with L-alanine dehydrogenase.
Synonyms
Alanine Racemase; EC 5.1.1.1; 9024-06-0; L-alanine racemase
