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Native Bacillus stearothermophilus Leucine Dehydrogenase

Cat No.
NATE-1905
Description
In enzymology, a leucine dehydrogenase (EC 1.4.1.9) is an enzyme that catalyzes the chemical reaction: L-leucine + H2O + NAD+ ↔ 4-methyl-2-oxopentanoate + NH3 + NADH + H+. The 3 substrates of this enzyme are L-leucine, H2O, and NAD+, whereas its 4 products are 4-methyl-2-oxopentanoate, NH3, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. This enzyme participates in valine, leucine and isoleucine degradation and valine, leucine and isoleucine biosynthesis.
Abbr
LeuDH, Native (Bacillus stearothermophilus)
Alias
LeuDH
Source
Bacillus stearothermophilus
Applications
The enzyme is useful for determination of L-leucine, L-valine or L-isoleucine.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.4.1.9
CAS No.
9082-71-7
Contaminants
(as LeuDH activity = 100 %)
NADH oxidase: < 0.01 %;
Lactate dehydrogenase: < 0.01 %.
Molecular Weight
ca. 300,000; Subunit molecular weight : ca. 49,000.
pH Stability
6.0 - 11.5
Michaelis Constant
(125mM Sodium phosphate buffer, pH 10.5, at 30 °C)
L-Leucine: 3.4 mM;
NAD+: 0.3 mM.
Specificity
L-Leucine: 100 %;
L-Valine : 86 %;
L-Isoleucine: 73 %.
Unit Definition
One unit of activity is defined as the amount of LeuDH that forms 1 μmol of NADH per minute at 30 °C
Optimum pH
10.6
Thermal stability
No detectable decrease in activity up to 60 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
EC 1.4.1.9; Leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); L-leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); LeuDH
Specific Activity
more than 40 U/mg protein
Reaction
L-Leucine + NAD+ + H2O ←→ α-Ketoisocaproate+ NH4+ +NADH
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1715
EC No.EC 1.4.1.9
CAS No.9082-71-7
SourceMicroorganism
CatalogNATE-1805
EC No.EC 1.4.1.9
CAS No.9082-71-7
SourceE. coli
CatalogDIA-209
EC No.EC 1.4.1.9
CAS No.9082-71-7
SourceBacillus sp.
CatalogEXWM-1463
EC No.EC 1.4.1.9
CAS No.9082-71-7
Source
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Realted Protocols
leucine dehydrogenase -Enzymatic Assay Protocol
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