Description
Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.
Abbr
Pyruvate Kinase, Native (Bacillus stearothermophilus)
Source
Bacillus stearothermophilus
Applications
Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. 1 It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.
Enzyme Commission Number
EC 2.7.1.40
Activity
100-300 units/mg protein
Unit Definition
One unit will convert 1.0 μmole of phospho (enol)pyruvate to pyruvate per min at pH 7.2 at 30°C.
Buffer
Lyophilized powder containing Tris buffer salts, pH 8.5
Synonyms
Pyruvate kinase; EC 2.7.1.40; 9001-59-6; phosphoenolpyruvate kinase; phosphoenol transphosphorylase; pyruvate kinase (phosphorylating); fluorokinase; fluorokinase (phosphorylating); pyruvic kinase; pyruvate phosphotransferase; ATP:pyruvate 2-O-phosphotransferase
